Significant Suppression of CT Radiation-Induced DNA Damage in Normal Human Cells by the PrC-210 Radioprotector
Jermusek F, Benedict C, Dreischmeier E, Brand M, Uder M, Jeffery JJ, Ranallo FN, Fahl WE (2018)
Publication Type: Journal article
Publication year: 2018
Journal
Book Volume: 190
Pages Range: 133-141
Journal Issue: 2
DOI: 10.1667/RR14928.1
Abstract
While computed tomography (CT) is now commonly used and considered to be clinically valuable, significant DNA double-strand breaks (γ-H2AX foci) in white blood cells from adult and pediatric CT patients have been frequently reported. In this study to determine whether γ-H2AX foci and X-ray-induced naked DNA damage are suppressed by administration of the PrC-210 radioprotector, human blood samples were irradiated in a CT scanner at 50-150 mGy with or without PrC-210, and γ-H2AX foci were scored. X-ray-induced naked DNA damage was also studied, and the DNA protective efficacy of PrC-210 was compared against 12 other common "antioxidants." PrC-210 reduced CT radiation-induced γ-H2AX foci in white blood cells to near background ( P < 0.0001) at radiation doses of 50-150 mGy. PrC-210 was most effective among the 13 "antioxidants" in reducing naked DNA X-ray damage, and its addition at 30 s before an •OH pulse reduced to background the •OH insult that otherwise induced >95% DNA damage. A systemic PrC-210 dose known to confer 100% survival in irradiated mice had no discernible effect on micro-CT image signal-to-noise ratio and CT image integrity. PrC-210 suppressed DNA damage to background or near background in each of these assay systems, thus supporting its development as a radioprotector for humans in multiple radiation exposure settings.
Authors with CRIS profile
Michael Brand
Lehrstuhl für Diagnostische Radiologie
Michael Uder
Lehrstuhl für Diagnostische Radiologie
Involved external institutions
United States (USA) (US)How to cite
APA:
Jermusek, F., Benedict, C., Dreischmeier, E., Brand, M., Uder, M., Jeffery, J.J.,... Fahl, W.E. (2018). Significant Suppression of CT Radiation-Induced DNA Damage in Normal Human Cells by the PrC-210 Radioprotector. Radiation Research, 190(2), 133-141. https://doi.org/10.1667/RR14928.1
MLA:
Jermusek, Frank, et al. "Significant Suppression of CT Radiation-Induced DNA Damage in Normal Human Cells by the PrC-210 Radioprotector." Radiation Research 190.2 (2018): 133-141.
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