Conformational Dynamics of Herpesviral NEC Proteins in Different Oligomerization States

Diewald B, Socher E, Söldner C, Sticht H (2018)

Publication Type: Journal article

Publication year: 2018


Book Volume: 19

Journal Issue: 10

DOI: 10.3390/ijms19102908


All herpesviruses use a heterodimeric nuclear egress complex (NEC) to transport capsids out of host cell nuclei. Despite their overall similar structure, NECs may differ significantly in sequence between different viruses. Up to now, structural information is limited to isolated NEC heterodimers and to large hexagonal lattices made up of hexagonal ring-like structures (Hexagons). The present study aimed to expand the existing structural knowledge with information on the dynamics of NECs from different viruses and in different oligomerization states. For this task, comparative molecular dynamics simulations were performed of the free NEC heterodimers from three different viruses (HCMV (human cytomegalovirus), HSV-1 (herpes simplex virus 1), and PRV (pseudorabies virus)). In addition, higher oligomerization states comprising two or six NEC heterodimers were characterized for HCMV and HSV-1. The study revealed that the isolated NEC heterodimers from - (HSV-1, PRV) and -herpesviruses (HCMV) differ significantly in their dynamics, which can be attributed to a poorly conserved interface region between the NEC subdomains. These differences become smaller for higher oligomerization states, and both HCMV and HSV-1 individual Hexagons exhibit a common region of enhanced dynamics, which might be of functional relevance for the formation of curved vesicle structures or the recognition of hexameric capsid proteins.

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Diewald, B., Socher, E., Söldner, C., & Sticht, H. (2018). Conformational Dynamics of Herpesviral NEC Proteins in Different Oligomerization States. International Journal of Molecular Sciences, 19(10).


Diewald, Benedikt, et al. "Conformational Dynamics of Herpesviral NEC Proteins in Different Oligomerization States." International Journal of Molecular Sciences 19.10 (2018).

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