Functional Analysis and Fine Mapping of the 9p22.2 Ovarian Cancer Susceptibility Locus

Buckley MA, Woods NT, Tyrer JP, Mendoza-Fandino G, Lawrenson K, Hazelett DJ, Najafabadi HS, Gjyshi A, Carvalho RS, Lyra PC, Coetzee SG, Shen HC, Yang AW, Earp MA, Yoder SJ, Risch H, Chenevix-Trench G, Ramus SJ, Phelan CM, Coetzee GA, Noushmehr H, Hughes TR, Sellers TA, Goode EL, Pharoah PD, Gayther SA, Monteiro ANA, Chen YA, Fridley BL, Aben KKH, Kiemeney LA, Anton-Culver H, Ziogas A, Bruinsma F, Milne RL, Bandera EV, Giles GG, Bean YT, Pejovic T, Beckmann M, Hein A, Bjorge L, Fasching PA, Thomsen LCV, Kopperud RK, Bischof K, Bogdanova N, Doek T, Hillemanns P, Brinton LA, Wentzensen N, Yang H, Brooks-Wilson A, Bunker CH, Butzow R, Nevanlinna H, Pelttari LM, Campbell IG, Southey MC, Modugno F, Carty K, Glasspool R, Mcneish I, Paul J, Siddiqui N, Chang-Claude J, Rudolph A, Chang-Claude J, Cook LS, Cramer DW, Terry KL, Cunningham JM, Cybulski C, Gronwald J, Jakubowska A, Lubinski J, Dansonka-Mieszkowska A, Kupryjanczyk J, Rzepecka IK, Du Bois A, Harter P, Dicks E, Song H, Doherty JA, Rossing MA, Duerst M, Easton DF, Eccles DM, Edwards RP, Ekici AB, Fasching P, Gao YT, Milne RL, Gentry-Maharaj A, Giles GG, Goodman MT, Thompson PJ, Hasmad HN, Teo SH, Hildebrandt MAT, Wu X, Hogdall E, Jensen A, Kjaer SK, Hogdall E, Iversen ES, Karlan BY, Lester J, Orsulic S, Walsh CS, Kelley JL, Lambrechts D, Lambrechts S, Vergote I, Lee AW, Levine DA, Liang D, Lissowska J, Lu K, Lundvall L, Kjaer SK, Massuger LFAG, Van Altena AM, Matsuo K, Mcguire V, Mclaughlin JR, Menon U, Moysich KB, Ness RB, Odunsi K, Olson SH, Orlow I, Pike MC, Pearce CL, Wu AH, Permuth JB, Tsai YY, Tworoger SS, Poole EM, Rosen B, Shu XO, Shvetsov YB, Wilkens LR, Sieh W, Spiewankiewicz B, Sucheston-Campbell L, Thomsen L, Wang-Gohrke S, Whittemore AS, Woo YL, Zheng W, Berchuck A, Chenevix-Trench G, Schildkraut JM, Kelemen LE, Freedman ML (2019)

Publication Type: Journal article

Publication year: 2019


Book Volume: 79

Journal Issue: 3

DOI: 10.1158/0008-5472.CAN-17-3864


Genome-wide association studies have identified 40 ovarian cancer risk loci. However, the mechanisms underlying these associations remain elusive. In this study, we conducted a two-pronged approach to identify candidate causal SNPs and assess underlying biological mechanisms at chromosome 9p22.2, the first and most statistically significant associated locus for ovarian cancer susceptibility. Three transcriptional regulatory elements with allele-specific effects and a scaffold/matrix attachment region were characterized and, through physical DNA interactions, BNC2 was established as the most likely target gene. We determined the consensus binding sequence for BNC2 in vitro, verified its enrichment in BNC2 ChIP-seq regions, and validated a set of its downstream target genes. Fine-mapping by dense regional genotyping in over 15,000 ovarian cancer cases and 30,000 controls identified SNPs in the scaffold/ matrix attachment region as among the most likely causal variants. This study reveals a comprehensive regulatory landscape at 9p22.2 and proposes a likely mechanism of susceptibility to ovarian cancer.

Authors with CRIS profile

Involved external institutions

University of New South Wales (UNSW) AU Australia (AU) H. Lee Moffitt Cancer Center & Research Institute US United States (USA) (US) University of California Irvine US United States (USA) (US) University of Toronto CA Canada (CA) Yale University US United States (USA) (US) Cancer Council Victoria AU Australia (AU) Cedars-Sinai Medical Center US United States (USA) (US) Radboud University Nijmegen NL Netherlands (NL) Stanford University US United States (USA) (US) Helsingin yliopisto / University of Helsinki FI Finland (FI) Ramsay Sime Darby Health Care (RSDHC) MY Malaysia (MY) Vanderbilt University US United States (USA) (US) Mayo Clinic US United States (USA) (US) University of Cambridge GB United Kingdom (GB) California State University (CSUF) US United States (USA) (US) University of Texas MD Anderson Cancer Center US United States (USA) (US) Danish Cancer Society Research Center DK Denmark (DK) Universität Ulm DE Germany (DE) Duke University US United States (USA) (US) University of Malaya (UM) / Universiti Malaya MY Malaysia (MY) The University of Melbourne AU Australia (AU) University College London (UCL) GB United Kingdom (GB) University of Hawaii (U.H.) US United States (USA) (US) University of Copenhagen DK Denmark (DK) Institute for Oncology Ljubljana (OIL) SI Slovenia (SI) Peter MacCallum Cancer Centre AU Australia (AU) Oregon Health and Science University (OSHU) US United States (USA) (US) Rutgers Cancer Institute of New Jersey US United States (USA) (US) Pomeranian Medical University / Pomorski Uniwersytet Medyczny w Szczecinie (PMU) PL Poland (PL) Deutsches Krebsforschungszentrum (DKFZ) DE Germany (DE) Kliniken Essen-Mitte DE Germany (DE) Beatson West of Scotland Cancer Centre (BWSCC) GB United Kingdom (GB) Haukeland University Hospital / Haukeland universitetssykehus NO Norway (NO) Medizinische Hochschule Hannover (MHH) / Hannover Medical School DE Germany (DE) University of Pittsburgh US United States (USA) (US) Maria Skłodowska-Curie Institute of Oncology / Centrum Onkologii–Instytut im. Marii Skłodowskiej-Curie w Warszawie PL Poland (PL) Shanghai Cancer Institute / 上海市肿瘤研究所 CN China (CN) National Cancer Institute (NCI) US United States (USA) (US) University of New Mexico (UNM) / Universidad de Nuevo México US United States (USA) (US) Friedrich-Schiller-Universität Jena DE Germany (DE) Brigham and Women's Hospital (BWH) US United States (USA) (US) Dartmouth College US United States (USA) (US) Princess Anne Hospital GB United Kingdom (GB) Glasgow Royal Infirmary (GRI) GB United Kingdom (GB) Katholieke Universiteit Leuven (KUL) / Catholic University of Leuven BE Belgium (BE) Dana–Farber Cancer Institute US United States (USA) (US) Roswell Park Cancer Institute US United States (USA) (US) Public Health Ontario CA Canada (CA) Medical University of South Carolina (MUSC) US United States (USA) (US) University of Virginia (UVA) US United States (USA) (US) University of Southern California (USC) US United States (USA) (US) QIMR Berghofer Medical Research Institute (früher: the Queensland Institute of Medical Research) AU Australia (AU) Memorial Sloan Kettering Cancer Center US United States (USA) (US) Texas Southern University (TSU) US United States (USA) (US) Ohio State University US United States (USA) (US) Henry Ford Health System (HFHS) US United States (USA) (US) Aichi Cancer Center Research Institute JP Japan (JP) British Columbia Cancer Agency CA Canada (CA) Icahn School of Medicine at Mount Sinai US United States (USA) (US) New York University (NYU) US United States (USA) (US) University of Washington US United States (USA) (US)

How to cite


Buckley, M.A., Woods, N.T., Tyrer, J.P., Mendoza-Fandino, G., Lawrenson, K., Hazelett, D.J.,... Freedman, M.L. (2019). Functional Analysis and Fine Mapping of the 9p22.2 Ovarian Cancer Susceptibility Locus. Cancer Research, 79(3).


Buckley, Melissa A., et al. "Functional Analysis and Fine Mapping of the 9p22.2 Ovarian Cancer Susceptibility Locus." Cancer Research 79.3 (2019).

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