The altered expression levels of miR-186, miR-494 and miR-3651 in OSCC tissue vary from those of the whole blood of OSCC patients

Ries J, Baran C, Wehrhan F, Weber M, Motel C, Kesting MR, Nkenke E (2018)

Publication Type: Journal article, Original article

Publication year: 2018

Journal

Abstract

BACKGROUND: Altered expressions of miR-186, miR-494 and miR-3651 in whole blood of OSCC patients have already been demonstrated. However, nothing is known about their expression in tumor tissues. This study aimed at evaluating differences in miRNA expression in OSCC tissues compared to healthy oral mucosa and at checking if the altered expression is reflected in corresponding blood. METHODS: In 53 OSCC and 40 healthy mucosal tissues the expression of miR-186, -494 and -3651 was determined by RT-qPCR and expression levels were compared between the groups. The altered expression in tissues was compared with that determined for corresponding blood. MiR-3651 target genes were identified using TargetScan software. RESULTS: Differential expression of miR-186 and miR-494 could not be observed in tumor tissues compared to normal mucosa (pmiR-186= 0.13; pmiR-494= 0.35). Contrary to the detected upregulation of the miR-3651 in the blood of OSCC patients, a significant downregulation was observed in OSCC tissues. A significant correlation between underexpression and diagnosis was ascertained (p= 0.0001). 1710 possible target genes of miR-3651 were identified. CONCLUSIONS: Decreased expression of miR-3651 in OSCC tissues may be a diagnostic biomarker. The opposite change in expression level in the blood might indicate different functions of this miRNA in circulation and tissue.

Authors with CRIS profile

Jutta Ries Medizinische Fakultät Christoph Baran Department of Oral and Cranio-Maxillofacial Surgery Falk Wehrhan Department of Oral and Cranio-Maxillofacial Surgery Manuel Weber Department of Oral and Cranio-Maxillofacial Surgery Constantin Motel Department of Prosthodontics Marco Rainer Kesting Lehrstuhl für Zahnmedizin, insbesondere Mund-, Kiefer-, und Gesichtschirurgie

Involved external institutions

Medizinische Universität Wien

Austria (AT)

How to cite

APA:

Ries, J., Baran, C., Wehrhan, F., Weber, M., Motel, C., Kesting, M.R., & Nkenke, E. (2018). The altered expression levels of miR-186, miR-494 and miR-3651 in OSCC tissue vary from those of the whole blood of OSCC patients. Cancer Biomarkers. https://doi.org/10.3233/CBM-180032

MLA:

Ries, Jutta, et al. "The altered expression levels of miR-186, miR-494 and miR-3651 in OSCC tissue vary from those of the whole blood of OSCC patients." Cancer Biomarkers (2018).

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