PiezoGRIN: A High-Pressure Chamber Incorporating GRIN Lenses for High-Resolution 3D-Microscopy of living Cells and Tissues

Schneidereit D, Schürmann S, Friedrich O (2018)

Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2018

Journal

Advanced Science Wiley Open Access

Publisher: John Wiley and Sons Inc.

Article Number: 1801453

DOI: 10.1002/advs.201801453

Abstract

A high‐pressure optical chamber, PiezoGRIN, that facilitates label‐free 3D high‐resolution live‐cell multiphoton microscopy in thick tissue samples is presented. A set of two Gradient Index (GRIN) rod lenses is integrated into the chamber as an optical guide and allows for the adjustment of the focal plane through the sample providing a field of view volume of 450 × 450 × 500 µm (x, y, z). An optical lateral resolution of 0.8 µm is achieved by using two‐photon excitation with 150 fs pulses of a 810 nm titanium–sapphire laser at hydrostatic pressures up to 200 MPa. With the PiezoGRIN setup, it is possible to follow pressure‐induced changes in subcellular structure of unstained vital mouse skeletal muscle tissue up to 200 µm below the tissue surface.

Authors with CRIS profile

Dominik Schneidereit Lehrstuhl für Medizinische Biotechnologie (MBT) Sebastian Schürmann Lehrstuhl für Medizinische Biotechnologie (MBT) Oliver Friedrich Lehrstuhl für Medizinische Biotechnologie (MBT)

Related research project(s)

Skeletal Muscle Function under Pressure: Elucidating Mechanisms of Muscle Ca2+ Signaling Failure and Ultrastructure Disintegration using a novel High-Pressure Multiphoton Microscopy Technology (PiezoGRIN) March 1, 2014 - July 31, 2018

How to cite

APA:

Schneidereit, D., Schürmann, S., & Friedrich, O. (2018). PiezoGRIN: A High-Pressure Chamber Incorporating GRIN Lenses for High-Resolution 3D-Microscopy of living Cells and Tissues. Advanced Science. https://doi.org/10.1002/advs.201801453

MLA:

Schneidereit, Dominik, Sebastian Schürmann, and Oliver Friedrich. "PiezoGRIN: A High-Pressure Chamber Incorporating GRIN Lenses for High-Resolution 3D-Microscopy of living Cells and Tissues." Advanced Science (2018).

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