Schneidereit D, Nübler S, Prölß G, Reischl B, Schürmann S, Müller OJ, Friedrich O (2018)
Publication Language: English
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 2018
Publisher: Nature Publishing Group
Book Volume: 7
Article Number: 79
Journal Issue: 1
DOI: 10.1038/s41377-018-0080-3
Skeletal muscle is an archetypal organ whose structure is tuned to match
function. The magnitude of order in muscle fibers and myofibrils
containing motor protein polymers determines the directed force output
of the summed force vectors and, therefore, the muscle’s power
performance on the structural level. Structure and function can change
dramatically during disease states involving chronic remodeling.
Cellular remodeling of the cytoarchitecture has been pursued using
noninvasive and label-free multiphoton second harmonic generation (SHG)
microscopy. Hereby, structure parameters can be extracted as a measure
of myofibrillar order and thus are suggestive of the force output that a
remodeled structure can still achieve. However, to date, the parameters
have only been an indirect measure, and a precise calibration of
optical SHG assessment for an exerted force has been elusive as no
technology in existence correlates these factors. We engineered a
novel, automated, high-precision biomechatronics system into a
multiphoton microscope allows simultaneous isometric Ca2+-graded force or passive viscoelasticity measurements and SHG recordings. Using this MechaMorph system, we studied force and SHG in single EDL muscle fibers from wt and mdx mice; the latter serves as a model for compromised force and abnormal myofibrillar structure. We present Ca2+-graded
isometric force, pCa-force curves, passive viscoelastic parameters and
3D structure in the same fiber for the first time. Furthermore, we
provide a direct calibration of isometric force to morphology, which
allows noninvasive prediction of the force output of single fibers from
only multiphoton images, suggesting a potential application in the
diagnosis of myopathies.
APA:
Schneidereit, D., Nübler, S., Prölß, G., Reischl, B., Schürmann, S., Müller, O.J., & Friedrich, O. (2018). Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach. Light: Science & Applications, 7(1). https://doi.org/10.1038/s41377-018-0080-3
MLA:
Schneidereit, Dominik, et al. "Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach." Light: Science & Applications 7.1 (2018).
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