Functional Viability: Measurement of Synaptic Vesicle Pool Sizes

Wrosch JK, Groemer TW (2017)


Publication Type: Journal article

Publication year: 2017

Journal

Book Volume: 1601

Pages Range: 195-204

DOI: 10.1007/978-1-4939-6960-9_15

Abstract

Neurons and their function of conveying information across a chemical synapse are highly regulated systems. Impacts on their functional viability can occur independently from changes in morphology. Here we describe a method to assess the size of synaptic vesicle pools using live cell fluorescence imaging and a genetically encoded probe (pHluorin). Assessing functional parameters such as the size of synaptic vesicle pools can be a valuable addition to common assays of neuronal cell viability as they demonstrate that key cellular functions are intact.

Authors with CRIS profile

How to cite

APA:

Wrosch, J.K., & Groemer, T.W. (2017). Functional Viability: Measurement of Synaptic Vesicle Pool Sizes. Methods in Molecular Biology, 1601, 195-204. https://doi.org/10.1007/978-1-4939-6960-9_15

MLA:

Wrosch, Jana Katharina, and Teja W. Groemer. "Functional Viability: Measurement of Synaptic Vesicle Pool Sizes." Methods in Molecular Biology 1601 (2017): 195-204.

BibTeX: Download