rs2735383, located at a microRNA binding site in the 3'UTR of NBS1, is not associated with breast cancer risk

Liu J, Loncar I, Collee JM, Bolla MK, Dennis J, Michailidou K, Wang Q, Andrulis IL, Barile M, Beckmann M, Behrens S, Benitez J, Blomqvist C, Boeckx B, Bogdanova NV, Bojesen SE, Brauch H, Brennan P, Brenner H, Broeks A, Burwinkel B, Chang-Claude J, Chen ST, Chenevix-Trench G, Cheng CY, Choi JY, Couch FJ, Cox A, Cross SS, Cuk K, Czene K, Doerk T, Dos-Santos-Silva I, Fasching P, Figueroa J, Flyger H, Garcia-Closas M, Giles GG, Glendon G, Goldberg MS, Gonzalez-Neira A, Guenel P, Haiman CA, Hamann U, Hart SN, Hartman M, Hatse S, Hopper JL, Ito H, Jakubowska A, Kabisch M, Kang D, Kosma VM, Kristensen VN, Le Marchand L, Lee E, Li J, Lophatananon A, Lubinski J, Mannermaa A, Matsuo K, Milne RL, Neuhausen SL, Nevanlinna H, Orr N, Perez JIA, Peto J, Putti TC, Pylkas K, Radice P, Sangrajrang S, Sawyer EJ, Schmidt MK, Schneeweiss A, Shen CY, Shrubsole MJ, Shu XO, Simard J, Southey MC, Swerdlow A, Teo SH, Tessier DC, Thanasitthichai S, Tomlinson I, Torres D, Truong T, Tseng CC, Vachon C, Winqvist R, Wu AH, Yannoukakos D, Zheng W, Hall P, Dunning AM, Easton DF, Hooning MJ, Van Den Ouweland AMW, Martens JWM, Hollestelle A (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Book Volume: 6

DOI: 10.1038/srep36874

Abstract

NBS1, also known as NBN, plays an important role in maintaining genomic stability. Interestingly, rs2735383 G > C, located in a microRNA binding site in the 3'-untranslated region (UTR) of NBS1, was shown to be associated with increased susceptibility to lung and colorectal cancer. However, the relation between rs2735383 and susceptibility to breast cancer is not yet clear. Therefore, we genotyped rs2735383 in 1,170 familial non-BRCA1/2 breast cancer cases and 1,077 controls using PCR-based restriction fragment length polymorphism (RFLP-PCR) analysis, but found no association between rs2735383CC and breast cancer risk (OR = 1.214, 95% CI = 0.936-1.574, P = 0.144). Because we could not exclude a small effect size due to a limited sample size, we further analyzed imputed rs2735383 genotypes (r2 > 0.999) of 47,640 breast cancer cases and 46,656 controls from the Breast Cancer Association Consortium (BCAC). However, rs2735383CC was not associated with overall breast cancer risk in European (OR = 1.014, 95% CI = 0.969-1.060, P = 0.556) nor in Asian women (OR = 0.998, 95% CI = 0.905-1.100, P = 0.961). Subgroup analyses by age, age at menarche, age at menopause, menopausal status, number of pregnancies, breast feeding, family history and receptor status also did not reveal a significant association. This study therefore does not support the involvement of the genotype at NBS1 rs2735383 in breast cancer susceptibility.

Authors with CRIS profile

Involved external institutions

Erasmus University Medical Center (MC) Netherlands (NL) Erasmus University Rotterdam (EUR) / Erasmus Universiteit Rotterdam Netherlands (NL) University of Cambridge United Kingdom (GB) Mount Sinai Hospital (MSH) Canada (CA) European Institute of Oncology / Istituto Europeo di Oncologia (IEO) Italy (IT) Deutsches Krebsforschungszentrum (DKFZ) Germany (DE) Spanish National Cancer Research Centre / Centro Nacional de Investigaciones Oncológicas (CNIO) Spain (ES) Helsingin yliopisto / University of Helsinki Finland (FI) Flanders Institute for Biotechnology / Vlaams Instituut voor Biotechnologie (VIB) Belgium (BE) Medizinische Hochschule Hannover (MHH) / Hannover Medical School Germany (DE) Copenhagen University Hospital Denmark (DK) Antoni van Leeuwenhoek Netherlands (NL) University of Sheffield United Kingdom (GB) London School of Hygiene and Tropical Medicine United Kingdom (GB) National University of Singapore (NUS) Singapore (SG) University Hospital Leuven (UZ) / Universitaire ziekenhuizen Leuven Belgium (BE) The University of Melbourne Australia (AU) Aichi Cancer Center Research Institute Japan (JP) National Cancer Institute (NCI) United States (USA) (US) King’s College London United Kingdom (GB) Ruprecht-Karls-Universität Heidelberg Germany (DE) Vanderbilt University United States (USA) (US) The Institute of Cancer Research (ICR) United Kingdom (GB) Cancer Research Initiatives Foundation (CARIF) / Cancer Research Malaysia (CRM) Malaysia (MY) École Polytechnique - Université Paris-Saclay France (FR) Robert-Bosch-Krankenhaus Germany (DE) International Agency for Research on Cancer (IARC) France (FR) Changhua Christian Hospital Taiwan (TW) QIMR Berghofer Medical Research Institute (früher: the Queensland Institute of Medical Research) Australia (AU) Singapore National Eye Centre (SNEC) Singapore (SG) Seoul National University (SNU) / 서울대학교 Korea, Republic of (KR) Mayo Clinic United States (USA) (US) Karolinska Institute Sweden (SE) University of Edinburgh United Kingdom (GB) Cancer Council Victoria Australia (AU) McGill University Canada (CA) Oslo University Hospital / Oslo Universitetssykehus Rikshospitalet Norway (NO) University of Southern California (USC) United States (USA) (US) University of Warwick United Kingdom (GB) Nagoya University / 名古屋大学 Japan (JP) Istituto di ricovero e cura a carattere scientifico (IRCCS) Italy (IT) University of Oxford United Kingdom (GB) Pomeranian Medical University / Pomorski Uniwersytet Medyczny w Szczecinie (PMU) Poland (PL) University of Eastern Finland Finland (FI) City of Hope Medical Center United States (USA) (US) China Medical University (CMU) / 中國醫藥大學 Taiwan (TW) University of Hawaii (U.H.) United States (USA) (US) Hospital Monte Naranco Spain (ES) Oulun Yliopisto / University of Oulo Finland (FI) Université Laval (UL) Canada (CA) National Centre for Scientific Research (NCSR) "Demokritos" Greece (GR)

How to cite

APA:

Liu, J., Loncar, I., Collee, J.M., Bolla, M.K., Dennis, J., Michailidou, K.,... Hollestelle, A. (2016). rs2735383, located at a microRNA binding site in the 3'UTR of NBS1, is not associated with breast cancer risk. Scientific Reports, 6. https://doi.org/10.1038/srep36874

MLA:

Liu, Jingjing, et al. "rs2735383, located at a microRNA binding site in the 3'UTR of NBS1, is not associated with breast cancer risk." Scientific Reports 6 (2016).

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