Mast cells have no impact on cutaneous leishmaniasis severity and related Th2 differentiation in resistant and susceptible mice

Paul C, Wolff S, Zapf T, Raifer H, Feyerabend TB, Bollig N, Camara B, Trier C, Schleicher U, Rodewald HR, Lohoff M (2016)

Publication Type: Journal article

Publication year: 2016

Journal

European Journal of Immunology Wiley-VCH Verlag

Book Volume: 46

Pages Range: 114-21

Journal Issue: 1

DOI: 10.1002/eji.201545613

Abstract

The genus leishmania comprises different protozoan parasites which are causative agents of muco-cutaneous and systemic, potentially lethal diseases. After infection with the species Leishmania major, resistant mice expand Th1 cells which stimulate macrophages for Leishmania destruction. In contrast, susceptible mice generate Th2 cells which deactivate macrophages, leading to systemic spread of the pathogens. Th-cell differentiation is determined within the first days, and Th2 cell differentiation requires IL-4, whereby the initial IL-4 source is often unknown. Mast cells are potential sources of IL-4, and hence their role in murine leishmaniasis has previously been studied in mast cell-deficient Kit mutant mice, although these mice display immunological phenotypes beyond mast cell deficiency. We therefore readdressed this question by infecting Kit-independent mast cell-deficient mice that are Th1 (C57BL/6 Cpa(Cre) ) or Th2 (BALB/c Cpa(Cre) ) prone with L. major. Using different parasite doses and intra- or subcutaneous infection routes, the results demonstrate no role of mast cells on lesion size development, parasite load, immune cell phenotypes expanding in draining lymph nodes, and cytokine production during murine cutaneous leishmaniasis. Thus, other cell types such as ILCs or T cells have to be considered as primary source of Th2-driving IL-4.

Authors with CRIS profile

Ulrike Schleicher Institute of Microbiology – Clinical Microbiology, Immunology and Hygiene

Involved external institutions

Philipps-Universität Marburg DE Germany (DE) Deutsches Krebsforschungszentrum (DKFZ) DE Germany (DE)

How to cite

APA:

Paul, C., Wolff, S., Zapf, T., Raifer, H., Feyerabend, T.B., Bollig, N.,... Lohoff, M. (2016). Mast cells have no impact on cutaneous leishmaniasis severity and related Th2 differentiation in resistant and susceptible mice. European Journal of Immunology, 46(1), 114-21. https://doi.org/10.1002/eji.201545613

MLA:

Paul, Christoph, et al. "Mast cells have no impact on cutaneous leishmaniasis severity and related Th2 differentiation in resistant and susceptible mice." European Journal of Immunology 46.1 (2016): 114-21.

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