Binding properties of SUMO-interacting motifs (SIMs) in yeast

Jardin C, Horn A, Sticht H (2015)

Publication Type: Journal article

Publication year: 2015


Publisher: Springer Verlag (Germany)

Book Volume: 21

Pages Range: 50

Journal Issue: 3

DOI: 10.1007/s00894-015-2597-1


Small ubiquitin-like modifier (SUMO) conjugation and interaction play an essential role in many cellular processes. A large number of yeast proteins is known to interact non-covalently with SUMO via short SUMO-interacting motifs (SIMs), but the structural details of this interaction are yet poorly characterized. In the present work, sequence analysis of a large dataset of 148 yeast SIMs revealed the existence of a hydrophobic core binding motif and a preference for acidic residues either within or adjacent to the core motif. Thus the sequence properties of yeast SIMs are highly similar to those described for human. Molecular dynamics simulations were performed to investigate the binding preferences for four representative SIM peptides differing in the number and distribution of acidic residues. Furthermore, the relative stability of two previously observed alternative binding orientations (parallel, antiparallel) was assessed. For all SIMs investigated, the antiparallel binding mode remained stable in the simulations and the SIMs were tightly bound via their hydrophobic core residues supplemented by polar interactions of the acidic residues. In contrary, the stability of the parallel binding mode is more dependent on the sequence features of the SIM motif like the number and position of acidic residues or the presence of additional adjacent interaction motifs. This information should be helpful to enhance the prediction of SIMs and their binding properties in different organisms to facilitate the reconstruction of the SUMO interactome.

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How to cite


Jardin, C., Horn, A., & Sticht, H. (2015). Binding properties of SUMO-interacting motifs (SIMs) in yeast. Journal of Molecular Modeling, 21(3), 50.


Jardin, Christophe, Anselm Horn, and Heinrich Sticht. "Binding properties of SUMO-interacting motifs (SIMs) in yeast." Journal of Molecular Modeling 21.3 (2015): 50.

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