Effect of ion release from Cu-doped 45S5 Bioglass® on 3D endothelial cell morphogenesis

Staehli C, James-Bhasin M, Hoppe A, Boccaccini AR, Nazhat SN (2015)

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2015


Publisher: Elsevier

Book Volume: 19

Pages Range: 15-22

DOI: 10.1016/j.actbio.2015.03.009


Both silicate-based bioactive glasses and copper ions have demonstrated angiogenic activity and therefore represent promising bioinorganic agents for the promotion of vascularization in tissue-engineered scaffolds. This study examined the effect of ionic release products from 45S5 Bioglass® doped with 0 and 2.5 wt.% CuO (BG and Cu-BG respectively) on the formation of capillary-like networks by SVEC4-10 endothelial cells (ECs) seeded in a three-dimensional (3D) type I collagen matrix. Copper and silicon release following 24 h dissolution increased non-proportionally with Cu-BG concentration in cell culture medium, while calcium levels were decreased below the initial medium concentration. EC network length, connectivity, branching, quantified by means of a 3D morphometric image analysis method, as well as proliferation and metabolic activity were reduced in a dose-dependent fashion by BG and Cu-BG ionic release products. This reduction was less prominent for BG compared to an equivalent concentration of Cu-BG, which was attributed to a lower extent of silicon release and calcium consumption. Moreover, a CuCl2 dose equivalent to the highest concentration of Cu-BG exhibited no effect on ECs. In conclusion, while the previously reported pro-angiogenic activity of both Bioglass® and copper may not be reflected in a direct response of ECs, this study provides a maximum glass concentration for non-harmful angiogenic stimulation to be examined in future work.

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Staehli, C., James-Bhasin, M., Hoppe, A., Boccaccini, A.R., & Nazhat, S.N. (2015). Effect of ion release from Cu-doped 45S5 Bioglass® on 3D endothelial cell morphogenesis. Acta Biomaterialia, 19, 15-22. https://dx.doi.org/10.1016/j.actbio.2015.03.009


Staehli, Christoph, et al. "Effect of ion release from Cu-doped 45S5 Bioglass® on 3D endothelial cell morphogenesis." Acta Biomaterialia 19 (2015): 15-22.

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