Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1? expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro
Dimitrijevic M, Aleksic I, Vujic V, Stanojevic S, Pilipovic I, von Hörsten S, Leposavic G (2014)
Publication Type: Journal article
Publication year: 2014
Journal
Publisher: Springer Verlag (Germany)
Book Volume: 36
Pages Range: 9696
Journal Issue: 4
DOI: 10.1007/s11357-014-9696-2
Abstract
In humans, usual aging, differently from successful aging, is associated with deregulation of proinflammatory/anti-inflammatory cytokine balance. The corresponding data from rat studies are limited. Therefore, we examined (i) cytokine messenger RNA (mRNA) profile of fresh peritoneal cells from 6- (adult), 24- (old), and 31-month-old (long-lived) AO rats and (ii) proinflammatory (IL-1? and IL-6) and anti-inflammatory (IL-10) cytokine, NO, and urea production in their LPS-stimulated cultures. Comparing with adult rats, cells from old ones expressed lower amount of TNF-? and IL-6 mRNAs, but greater amount of IL-1? mRNA. On the other hand, cells from long-lived rats exhibited a dramatic increase in IL-10 mRNA expression followed by diminished TNF-? and IL-6 mRNA expression, and comparable expression of IL-1? mRNA relative to adult rats. Consequently, IL-10/IL-1? mRNA ratio was greater in cells from long-lived rats than in adult and old rats. In LPS-stimulated peritoneal cell cultures (contained >=95 % macrophages) from old rats, concentration of common proinflammatory cytokines was higher than in those from adult rats. Comparing with adult and old rats, in LPS-stimulated macrophage cultures from long-lived rats, TNF-? and IL-6 concentrations were lower; IL-1? concentration was comparable or greater (in respect to adult rats), whereas that of IL-10 was strikingly higher. Consistently, in macrophage cultures from long-lived rats, NO (iNOS activity marker)/urea (arginase activity marker) ratio was less and not different from that in old and adult rats, respectively. The study suggests that macrophages from long-lived rats, differently from those of old ones, have substantial ability to limit proinflammatory mediator production, which may contribute to their longevity.
Authors with CRIS profile
Involved external institutions
Institute of Virology, Vaccines and Sera "Torlak" / Институт за вирусологију, вакцине и серуме „Торлак“
Serbia (RS)
University of Belgrade / Универзитет у Београду
Serbia (RS)
Serbia (RS)
University of Belgrade / Универзитет у Београду
Serbia (RS)
How to cite
APA:
Dimitrijevic, M., Aleksic, I., Vujic, V., Stanojevic, S., Pilipovic, I., von Hörsten, S., & Leposavic, G. (2014). Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1? expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro. Age, 36(4), 9696. https://doi.org/10.1007/s11357-014-9696-2
MLA:
Dimitrijevic, Mirjana, et al. "Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1? expression ratio and preserved NO/urea ratio following LPS-stimulation in vitro." Age 36.4 (2014): 9696.
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