T-cell subsets in autologous and allogeneic peripheral blood stem cell concentrates

Strobel J, Möllmer I, Zingsem J, Hauck-Dlimi B, Eckstein R, Strasser E (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Vox Sanguinis Wiley-Blackwell

Book Volume: 109

Pages Range: 375-86

Journal Issue: 4

DOI: 10.1111/vox.12289

Abstract

Regulatory T cells (Tregs) and other T-cell subsets are of importance in the setting of autologous and allogeneic stem cell transplantations. We conducted a study to assess the content of peripheral blood stem cell concentrates and related apheresis parameters in the autologous and allogeneic setting.We characterized 53 donors, patients and peripheral blood stem cell concentrates (PBSC) regarding the content of CD45(+) cells, lymphocytes, CD3(+) cells, CD3(+) CD4(+) T cells, CD3(+) CD4(+) CD25(+) T cells, CD3(+) CD4(+) CD25(+) CD127(low/negative) Tregs and CD34(+) cells and calculated cell yields, recruitment factors and collection efficiency for all cell types. We compared allogeneic data with autologous data.Autologous PBSC show significantly lower concentrations of T-cell subsets compared to allogeneic PBSC (17,112/?l CD4(+), 14,858/?l CD4(+) CD25(+) and 1579/?l CD3(+) CD4(+) CD25(+) CD127(low/negative) Tregs in autologous compared to 65,539/?l CD4(+), 44,208(+) /?l CD4(+) CD25(+) and 5040/?l CD3(+) CD4(+) CD25(+) CD127(low/negative) Tregs in allogeneic PBSC, respectively), in contrast to CD34(+) concentrations (5342/?l CD34(+) in autologous compared to 2367/?l CD34(+) in allogeneic PBSC, respectively). Accordantly, all T-cell yields are lower in the autologous setting compared to allogeneic PBSC. However, recruitment factor and collection efficiency of all cell types are higher in autologous compared to allogeneic PBSC, but not all parameters differ significantly when groups are compared.T-cell subsets and especially Tregs are a substantial part of PBSC transplantation, as considerable recruitment during apheresis occurs. In large volume apheresis, the collection efficiency of Treg is comparable to that of CD34(+) cells, while recruitment factors are even higher.

Authors with CRIS profile

Joachim Strobel Institut für Experimentelle und Klinische Pharmakologie und Toxikologie Ina Möllmer Professur für Transfusionsmedizin und Cell-Engineering Jürgen Zingsem Medizinische Fakultät Barbara Hauck-Dlimi Medizinische Fakultät Reinhold Eckstein Professur für Transfusionsmedizin und Cell-Engineering Erwin Strasser Medizinische Fakultät

How to cite

APA:

Strobel, J., Möllmer, I., Zingsem, J., Hauck-Dlimi, B., Eckstein, R., & Strasser, E. (2015). T-cell subsets in autologous and allogeneic peripheral blood stem cell concentrates. Vox Sanguinis, 109(4), 375-86. https://doi.org/10.1111/vox.12289

MLA:

Strobel, Joachim, et al. "T-cell subsets in autologous and allogeneic peripheral blood stem cell concentrates." Vox Sanguinis 109.4 (2015): 375-86.

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