Measuring average rheological quantities of cell monolayers in the linear viscoelastic regime

Dakhil H, Gilbert D, Malhotra D, Limmer A, Engelhardt H, Amtmann A, Hansmann J, Hübner H, Buchholz R, Friedrich O, Wierschem A (2016)

Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2016

Journal

Rheologica Acta Springer Verlag (Germany)

Publisher: Springer Verlag (Germany)

Book Volume: 55

Pages Range: 527-536

Journal Issue: 7

DOI: 10.1007/s00397-016-0936-5

Abstract

During the last decades, the rheology of cells has been studied almost entirely in single cells. While cell-to-cell variation is typically very large and most studies were carried out in the nonlinear viscoelastic regime, we quantify average linear viscoelastic cell properties like storage and loss moduli and normal stress in monolayers of different cell types showing that murine 3T6 fibroblasts, human fibroblasts, and HeLa cells differ considerably in their storage modulus. To this end, we modified a commercial rheometer to set up a parallel-disk configuration at gap widths of a few micrometers and optically detected the cell concentration in the gap. This enables studying the linear viscoelastic behavior of the cells and permits quantifying the impact of drugs affecting the cytoskeleton or the extracellular matrix connection. Thus, due to its high-content approach, without the need of treating the samples in the rheometer, this envisions the use of this method as a fast diagnostic tool. The method also allows for quantitatively studying of the impact of pre-stress on the storage and loss moduli of the cells.

Authors with CRIS profile

Haider Dakhil Professur für Hochdruckthermofluiddynamik und Rheologie Daniel Gilbert Lehrstuhl für Medizinische Biotechnologie (MBT) Deepika Malhotra Lehrstuhl für Chemische Reaktionstechnik (CRT) Hannes Engelhardt Lehrstuhl für Bioverfahrenstechnik Holger Hübner Lehrstuhl für Bioverfahrenstechnik Rainer Buchholz Technische Fakultät Oliver Friedrich Lehrstuhl für Medizinische Biotechnologie (MBT) Andreas Wierschem Professur für Hochdruckthermofluiddynamik und Rheologie

Additional Organisation(s)

Erlangen Graduate School in Advanced Optical Technologies (SAOT)

Involved external institutions

Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik IGB / Fraunhofer Institute for Interfacial Engineering and Biotechnology DE Germany (DE)

How to cite

APA:

Dakhil, H., Gilbert, D., Malhotra, D., Limmer, A., Engelhardt, H., Amtmann, A.,... Wierschem, A. (2016). Measuring average rheological quantities of cell monolayers in the linear viscoelastic regime. Rheologica Acta, 55(7), 527-536. https://doi.org/10.1007/s00397-016-0936-5

MLA:

Dakhil, Haider, et al. "Measuring average rheological quantities of cell monolayers in the linear viscoelastic regime." Rheologica Acta 55.7 (2016): 527-536.

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