Maintenance of a bone collagen phenotype by osteoblast-like cells in 3D periodic porous titanium (Ti-6Al-4 V) structures fabricated by selective electron beam melting

Hrabe NW, Heinl P, Bordia RK, Körner C, Fernandes RJ (2013)

Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2013

Journal

Connective Tissue Research Informa Healthcare

Book Volume: 54

Pages Range: 351-360

Journal Issue: 6

DOI: 10.3109/03008207.2013.822864

Abstract

Regular 3D periodic porous Ti-6Al-4V structures were fabricated by the selective electron beam melting method (EBM) over a range of relative densities (0.17-0.40) and pore sizes (500-1500m). Structures were seeded with human osteoblast-like cells (SAOS-2) and cultured for four weeks. Cells multiplied within these structures and extracellular matrix collagen content increased. Type I and type V collagens typically synthesized by osteoblasts were deposited in the newly formed matrix with time in culture. High magnification scanning electron microscopy revealed cells attached to surfaces on the interior of the structures with an increasingly fibrous matrix. The in-vitro results demonstrate that the novel EBM-processed porous structures, designed to address the effect of stress-shielding, are conducive to osteoblast attachment, proliferation and deposition of a collagenous matrix characteristic of bone. © 2013 Informa Healthcare USA, Inc.

Authors with CRIS profile

Peter Heinl Lehrstuhl für Werkstoffwissenschaften (Werkstoffkunde und Technologie der Metalle) (WTM) Carolin Körner Lehrstuhl für Werkstoffwissenschaften (Werkstoffkunde und Technologie der Metalle) (WTM)

Related research project(s)

Activity SEBM of special alloys Jan. 1, 2000 - Jan. 1, 2003

Involved external institutions

University of Washington US United States (USA) (US)

How to cite

APA:

Hrabe, N.W., Heinl, P., Bordia, R.K., Körner, C., & Fernandes, R.J. (2013). Maintenance of a bone collagen phenotype by osteoblast-like cells in 3D periodic porous titanium (Ti-6Al-4 V) structures fabricated by selective electron beam melting. Connective Tissue Research, 54(6), 351-360. https://doi.org/10.3109/03008207.2013.822864

MLA:

Hrabe, Nikolas W., et al. "Maintenance of a bone collagen phenotype by osteoblast-like cells in 3D periodic porous titanium (Ti-6Al-4 V) structures fabricated by selective electron beam melting." Connective Tissue Research 54.6 (2013): 351-360.

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