Interferon Gamma Counteracts the Angiogenic Switch and Induces Vascular Permeability in Dextran Sulfate Sodium Colitis in Mice

Haep L, Britzen-Laurent N, Weber TG, Naschberger E, Schäfer A, Kremmer E, Försch S, Scheuer W, Wirtz S, Waldner M, Stürzl M, Vieth M (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Inflammatory Bowel Diseases Wiley

Book Volume: 21

Pages Range: 2360-71

Journal Issue: 10

DOI: 10.1097/MIB.0000000000000490

Abstract

Interferon (IFN)-? is a central pathogenesis factor in inflammatory bowel disease (IBD) with pleiotropic effects on many different cell types. However, as yet, the immune modulatory functions of IFN-? in IBD have been predominantly investigated. Based on previous studies showing that IFN-? acts antiangiogenic in colorectal carcinoma, we investigated the effects of IFN-? on the vascular system in IBD.Colon tissues of patients with IBD and dextran sulfate sodium-induced colitis in mice were subjected to immunohistochemistry, quantitative real-time polymerase chain reactions, and in situ hybridization to quantify cell activation, angiogenesis, and immune responses. Vascular structure and permeability in mice were analyzed by ultramicroscopy and in vivo confocal laser endomicroscopy.We showed a significantly increased blood vessel density in IBD and dextran sulfate sodium colitis. In mice, this was associated with a disorganized blood vessel structure and profound vascular leakage. As compared with genes associated with angiogenesis, genes associated with inflammatory cell activation including IFN-? were more strongly upregulated in colitis tissues. IFN-? exerted direct effects on endothelial cells in IBD tissues in vivo, as indicated by the expression of IFN-?-induced guanylate binding protein 1 (GBP-1). Neutralization of IFN-? in the acute dextran sulfate sodium colitis model demonstrated that this cytokine exerts endogenous angiostatic activity in IBD and contributes to increased vascular permeability.The dissection of the pleiotropic activities of IFN-? in IBD provides new insights to the pathological functions of this cytokine and may be of high relevance for the optimization of combination therapy approaches.

Authors with CRIS profile

Lisa Haep Professur für Molekulare und Experimentelle Chirurgie Nathalie Britzen-Laurent Professur für Molekulare und Experimentelle Chirurgie Elisabeth Naschberger Medizinische Fakultät Amrei Schäfer Department of Paediatric Cardiology Stefan Wirtz Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology Maximilian Waldner Lehrstuhl für Innere Medizin I Michael Stürzl Professur für Molekulare und Experimentelle Chirurgie Michael Vieth

Involved external institutions

Roche Deutschland Holding GmbH DE Germany (DE) Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE) Klinikum Bayreuth DE Germany (DE)

How to cite

APA:

Haep, L., Britzen-Laurent, N., Weber, T.G., Naschberger, E., Schäfer, A., Kremmer, E.,... Vieth, M. (2015). Interferon Gamma Counteracts the Angiogenic Switch and Induces Vascular Permeability in Dextran Sulfate Sodium Colitis in Mice. Inflammatory Bowel Diseases, 21(10), 2360-71. https://doi.org/10.1097/MIB.0000000000000490

MLA:

Haep, Lisa, et al. "Interferon Gamma Counteracts the Angiogenic Switch and Induces Vascular Permeability in Dextran Sulfate Sodium Colitis in Mice." Inflammatory Bowel Diseases 21.10 (2015): 2360-71.

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