Structural analysis of a plant sucrose carrier using monoclonal antibodies and bacteriophage lambda surface display.
Stolz J, Ludwig A, Stadler R, Biesgen C, Hagemann K, Sauer N (1999)
Publication Language: English
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 1999
Journal
Book Volume: 453
Pages Range: 375-379
Journal Issue: 3
DOI: 10.1016/S0014-5793(99)00756-5
Open Access Link: http://onlinelibrary.wiley.com/doi/10.1016/S0014-5793(99)00756-5/abstract
Abstract
Monoclonal antibodies were raised and selected against recombinant Plantago major PmSUC2 sucrose carrier protein. Epitopes of two monoclonal antibodies (PS2-1A2 and PS2-4D4) were mapped using N-terminally truncated PmSUC2 proteins and a lambda library displaying random PmSUC2 peptides. PS2-1A2 recognizes an octapeptide close to the N-terminus of PmSUC2, PS2-4D4 binds to a decapeptide at the very C-terminus. Analyses of antibody binding to yeast protoplasts with functionally active, tagged PmSUC2 protein revealed that both epitopes are located in cytoplasmic domains of PmSUC2. These results support a model for plant sucrose transporters containing 12 transmembrane helices with the N-terminus and the C-terminus on the cytoplasmic side of the plasma membrane.
Authors with CRIS profile
Ruth Stadler
Lehrstuhl für Molekulare Pflanzenphysiologie
Norbert Sauer
Lehrstuhl für Molekulare Pflanzenphysiologie
Involved external institutions
Germany (DE)How to cite
APA:
Stolz, J., Ludwig, A., Stadler, R., Biesgen, C., Hagemann, K., & Sauer, N. (1999). Structural analysis of a plant sucrose carrier using monoclonal antibodies and bacteriophage lambda surface display. Febs Letters, 453(3), 375-379. https://doi.org/10.1016/S0014-5793(99)00756-5
MLA:
Stolz, Jürgen, et al. "Structural analysis of a plant sucrose carrier using monoclonal antibodies and bacteriophage lambda surface display." Febs Letters 453.3 (1999): 375-379.
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