Behavior of encapsulated MG-63 cells in RGD and gelatine-modified alginate hydrogels

Grigore A, Sarker B, Fabry B, Boccaccini AR, Detsch R (2014)

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2014


Book Volume: 20

Pages Range: 2140-2150

DOI: 10.1089/ten.tea.2013.0416


Achieving cell spreading and proliferation inside hydrogels that are compatible with microencapsulation technology represents a major challenge for tissue engineering scaffolding and for the development of three-dimensional cell culture models. In this study, microcapsules of 650-900μm in diameter were fabricated from oxidized alginate covalently cross-linked with gelatine (AlGel). Schiff's base bond formed in AlGel, detected by Fourier transform infrared spectroscopy, which confirmed the cross-linking of oxidized alginate with gelatine. Biological properties of alginate based hydrogels were studied by comparing the viability and morphology of MG-63 osteosarcoma cells encapsulated in gelatine and RGD-modified alginate. We hypothesized that the presence of gelatine and RGD will support cell adhesion and spreading inside the microcapsules and finally, also vascular endothelial growth factor (VEGF) secretion. After 4 days of incubation, cells formed extensive cortical protrusions and after 2 weeks they proliferated, migrated, and formed cellular networks through the AlGel material. In contrast, cells encapsulated in pure alginate and in RGD-modified alginate formed spherical aggregates with limited cell mobility and VEGF secretion. Metabolic activity was doubled after 5 days of incubation, making AlGel a promising material for cell encapsulation. © Mary Ann Liebert, Inc.

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Grigore, A., Sarker, B., Fabry, B., Boccaccini, A.R., & Detsch, R. (2014). Behavior of encapsulated MG-63 cells in RGD and gelatine-modified alginate hydrogels. Tissue Engineering - Part A, 20, 2140-2150.


Grigore, Alexandra, et al. "Behavior of encapsulated MG-63 cells in RGD and gelatine-modified alginate hydrogels." Tissue Engineering - Part A 20 (2014): 2140-2150.

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