Loss of cytosolic fructose-1,6-bisphosphatase limits photosynthetic sucrose synthesis and causes severe growth retardations in rice (Oryza sativa)

Lee SK, Jeon JS, Börnke F, Voll L, Cho JI, Goh CH, Jeong SW, Park YI, Kim SJ, Choi SB, Miyao A, Hirochika H, An G, Cho MH, Bhoo SH, Sonnewald U, Hahn TR (2008)

Publication Status: Published

Publication Type: Journal article

Publication year: 2008

Journal

Plant Cell and Environment Wiley-Blackwell

Publisher: WILEY-BLACKWELL PUBLISHING, INC

Book Volume: 31

Pages Range: 1851-1863

Journal Issue: 12

DOI: 10.1111/j.1365-3040.2008.01890.x

Abstract

During photosynthesis, triose-phosphates (trioseP) exported from the chloroplast to the cytosol are converted to sucrose via cytosolic fructose-1,6-bisphosphatase (cFBPase). Expression analysis in rice suggests that OscFBP1 plays a major role in the cytosolic conversion of trioseP to sucrose in leaves during the day. The isolated OscFBP1 mutants exhibited markedly decreased photosynthetic rates and severe growth retardation with reduced chlorophyll content, which results in plant death. Analysis of primary carbon metabolites revealed both significantly reduced levels of sucrose, glucose, fructose and starch in leaves of these mutants, and a high accumulation of sucrose to starch in leaves of rice plants. In the oscfbp1 mutants, products of glycolysis and the TCA cycle were significantly increased. A partitioning experiment of (14)C-labelled photoassimilates revealed altered carbon distributions including a slight increase in the insoluble fraction representing transitory starch, a significant decrease in the neutral fraction corresponding to soluble sugars and a high accumulation of phosphorylated intermediates and carboxylic acid fractions in the oscfbp1 mutants. These results indicate that the impaired synthesis of sucrose in rice cannot be sufficiently compensated for by the transitory starch-mediated pathways that have been found to facilitate plant growth in the equivalent Arabidopsis mutants.

Authors with CRIS profile

Frederik Börnke Lehrstuhl für Biochemie Lars Voll Lehrstuhl für Biochemie Uwe Sonnewald Lehrstuhl für Biochemie

Involved external institutions

Kyung Hee University KR Korea, Republic of (KR) Jeju National University KR Korea, Republic of (KR) Chungnam National University (CNU) KR Korea, Republic of (KR) Myongji University KR Korea, Republic of (KR) National Institute of Agrobiological Sciences (NIAS) JP Japan (JP) Pohang University of Science and Technology (POSTECH) KR Korea, Republic of (KR)

How to cite

APA:

Lee, S.-K., Jeon, J.-S., Börnke, F., Voll, L., Cho, J.-I., Goh, C.-H.,... Hahn, T.-R. (2008). Loss of cytosolic fructose-1,6-bisphosphatase limits photosynthetic sucrose synthesis and causes severe growth retardations in rice (Oryza sativa). Plant Cell and Environment, 31(12), 1851-1863. https://doi.org/10.1111/j.1365-3040.2008.01890.x

MLA:

Lee, Sang-Kyu, et al. "Loss of cytosolic fructose-1,6-bisphosphatase limits photosynthetic sucrose synthesis and causes severe growth retardations in rice (Oryza sativa)." Plant Cell and Environment 31.12 (2008): 1851-1863.

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