Sonnewald U (2004)
Publication Status: Published
Publication Type: Journal article
Publication year: 2004
Publisher: FRONTIERS IN BIOSCIENCE INC
Book Volume: 9
Pages Range: 1803-1816
The cloning of the genes involved in the purine biosynthesis pathway was attained by a screening strategy with heterologous cDNA probes and by using S. cerevisiae mutants for complementation. Southern hybridization showed a complex genomic organization for these genes in solanaceous species and their organ- and developmental specific expression was analyzed by Northern hybridization. The specific role of ATase for plant growth and development was analyzed in transgenic tobacco plants exhibiting a reduced ATase activity and in an Arabidopsis T-DNA mutant (atd2) deficient for ATase2. The transgenic tobacco plants as well as the Arabidopsis mutant exhibit a specific and comparable phenotype, which is characterized by strong growth retardation and severe chlorosis in leaves. The formation of white leaves, but green cotyledons is a characteristic trait of the Arabidopsis atd2 mutant.
APA:
Sonnewald, U. (2004). Molecular analysis of "de novo" purine biosynthesis in solanaceous species and in Arabidopsis thaliana. Frontiers in Bioscience, 9, 1803-1816.
MLA:
Sonnewald, Uwe. "Molecular analysis of "de novo" purine biosynthesis in solanaceous species and in Arabidopsis thaliana." Frontiers in Bioscience 9 (2004): 1803-1816.
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