Application of surface plasmon resonance imaging technique for the detection of single spherical biological submicrometer particles

Shpacovitch V, Temchura V, Matrosovich M, Hamacher J, Skolnik J, Libuschewski P, Siedhoff D, Weichert F, Marvvedel P, Mueller H, Überla K, Hergenroeder R, Zybin A (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Analytical Biochemistry Elsevier Masson

Book Volume: 486

Pages Range: 62-9

DOI: 10.1016/j.ab.2015.06.022

Abstract

Recent proof-of-principle studies demonstrated the suitability of the surface plasmon resonance imaging (SPRi) technique for the detection of individual submicrometer and nanoparticles in solutions. In the current study, we used the SPRi technique for visualization of the binding of round-shaped viruses (inactivated influenza A virus) and virus-like particles (human immunodeficiency virus (HIV)-based virus-like particles) to the functionalized sensor surface. We show the applicability of the SPRi technique for the detection of individual virus-like particles in buffers without serum as well as in buffers containing different concentrations of serum. Furthermore, we prove the specificity of visualized binding events using two different pseudotypes of HIV virus-like particles. We also demonstrate the applicability of the SPRi technique for the determination of relative particle concentrations in solutions. Moreover, we suggest a technical approach, which allows enhancing the magnitude of binding signals. Our studies indicate that the SPRi technique represents an efficient research tool for quantification and characterization of biological submicrometer objects such as viruses or virus-like particles, for example.

Authors with CRIS profile

Vladimir Temchura Institute of Clinical and Molecular Virology Klaus Überla Institute of Clinical and Molecular Virology

Involved external institutions

Leibniz-Institut für Analytische Wissenschaften / Leibniz Institute for Analytical Sciences (ISAS) DE Germany (DE) Technische Universität Dortmund DE Germany (DE) Rheinische Friedrich-Wilhelms-Universität Bonn DE Germany (DE) Philipps-Universität Marburg DE Germany (DE)

How to cite

APA:

Shpacovitch, V., Temchura, V., Matrosovich, M., Hamacher, J., Skolnik, J., Libuschewski, P.,... Zybin, A. (2015). Application of surface plasmon resonance imaging technique for the detection of single spherical biological submicrometer particles. Analytical Biochemistry, 486, 62-9. https://doi.org/10.1016/j.ab.2015.06.022

MLA:

Shpacovitch, Victoria, et al. "Application of surface plasmon resonance imaging technique for the detection of single spherical biological submicrometer particles." Analytical Biochemistry 486 (2015): 62-9.

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