Spinach ferredoxin is a calcium-binding protein

Surek B, Kreimer G, Melkonian M, Latzko E (1987)

Publication Status: Published

Publication Type: Journal article

Publication year: 1987


Publisher: Springer Verlag (Germany)

Book Volume: 171

Pages Range: 565-568

Journal Issue: 4

DOI: 10.1007/BF00392307


Spinach-leaf ferredoxin was identified as a calcium-binding protein by 45Ca autoradiography on nitrocellulose membranes and with the cationic carbocyanine dye 1-ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl] naphtho[1,2-d]thiazolium bromide (“stains-all”). Binding of 45Ca was observed at pH 6.8 and pH 7.8 and in the presence of 5 mM and 20 mM MgCl2. At the higher MgCl2 concentration the Ca2+-binding capacity is reduced. Only micromolar concentrations of LaCl3, however, are required to achieve a similar effect. Both the oxidized and reduced forms of ferredoxin bind calcium.

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Surek, B., Kreimer, G., Melkonian, M., & Latzko, E. (1987). Spinach ferredoxin is a calcium-binding protein. Planta, 171(4), 565-568. https://dx.doi.org/10.1007/BF00392307


Surek, Barbara, et al. "Spinach ferredoxin is a calcium-binding protein." Planta 171.4 (1987): 565-568.

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