Surface Trafficking of APP and BACE in Live Cells
Bauereiß A, Welzel O, Jung J, Grosse-Holz S, Lelental N, Lewczuk P, Wenzel EM, Kornhuber J, Groemer TW (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 16
Pages Range: 655-75
Journal Issue: 6
DOI: 10.1111/tra.12270
Abstract
Amyloid-? (A?)-peptide, the major constituent of the plaques that develop during Alzheimer's disease, is generated via the cleavage of A? precursor protein (APP) by ?-site APP-cleaving enzyme (BACE). Using live-cell imaging of APP and BACE labeled with pH-sensitive proteins, we could detect the release events of APP and BACE and their distinct kinetics. We provide kinetic evidence for the cleavage of APP by ?-secretase on the cellular surface after exocytosis. Furthermore, simultaneous dual-color evanescent field illumination revealed that the two proteins are trafficked to the surface in separate compartments. Perturbing the membrane lipid composition resulted in a reduced frequency of exocytosis and affected BACE more strongly than APP. We propose that surface fusion frequency is a key factor regulating the aggregation of APP and BACE in the same membrane compartment and that this process can be modulated via pharmacological intervention.
Authors with CRIS profile
Anna Bauereiß
Lehrstuhl für Psychiatrie und Psychotherapie
Jasmin Jung
Lehrstuhl für Psychiatrie und Psychotherapie
Natalia Ermann
Medizinische Fakultät
Piotr Lewczuk
Department of Psychiatry and Psychotherapy
Johannes Kornhuber
Lehrstuhl für Psychiatrie und Psychotherapie
Involved external institutions
Norway (NO)How to cite
APA:
Bauereiß, A., Welzel, O., Jung, J., Grosse-Holz, S., Lelental, N., Lewczuk, P.,... Groemer, T.W. (2015). Surface Trafficking of APP and BACE in Live Cells. Traffic, 16(6), 655-75. https://doi.org/10.1111/tra.12270
MLA:
Bauereiß, Anna, et al. "Surface Trafficking of APP and BACE in Live Cells." Traffic 16.6 (2015): 655-75.
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