Starke C, Frey S, Wellmann U, Urbonaviciute V, Herrmann M, Amann KU, Schett G, Winkler T, Voll RE (2011)
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 2011
Publisher: WILEY-BLACKWELL
Book Volume: 41
Pages Range: 2107-2112
Journal Issue: 7
Autoantibodies to double-stranded (ds) DNA represent a serological hallmark of systemic lupus erythematosus (SLE) and may critically contribute to the pathogenesis of lupus nephritis. Self-reactive antibodies might be partially produced by long-lived plasma cells (PCs), which mainly reside within the bone marrow and spleen. In contrast to short-lived PCs, long-lived PCs are extremely resistant to therapy and may sustain refractory disease courses. Recently, antibody-secreting cells were found within the inflamed kidneys of New Zealand black/white (NZB/W) F1 lupus mice as well as of patients with SLE. To analyze the longevity of the IgG-producing cells present in nephritic kidneys of NZB/W F1 mice we performed in vivo BrdU-labeling. We identified a higher frequency of long-lived than short-lived renal PCs, indicating that survival niches for long-lived PCs also exist within inflamed kidneys. Using ELISPOT assays, we found that on average 31% of renal IgG-producing cells reacted with dsDNA and 24% with nucleolin. Moreover, the frequencies of IgG-secreting cells specific for the autoantigens dsDNA and nucleolin were higher in the kidneys compared with those in the spleen and bone marrow.
APA:
Starke, C., Frey, S., Wellmann, U., Urbonaviciute, V., Herrmann, M., Amann, K.U.,... Voll, R.E. (2011). High frequency of autoantibody-secreting cells and long-lived plasma cells within inflamed kidneys of NZB/W F1 lupus mice. European Journal of Immunology, 41(7), 2107-2112. https://doi.org/10.1002/eji.201041315
MLA:
Starke, Charlotte, et al. "High frequency of autoantibody-secreting cells and long-lived plasma cells within inflamed kidneys of NZB/W F1 lupus mice." European Journal of Immunology 41.7 (2011): 2107-2112.
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