Combination of extrinsic and intrinsic pathways significantly accelerates axial vascularization of bioartificial tissues

Arkudas A, Galyna P, Beier J, Weigel L, Körner C, Singer R, Bleiziffer O, Polykandriotis E, Horch RE, Kneser U (2012)

Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2012

Journal

Book Volume: 129

Pages Range: 55e-65e

Journal Issue: 1

DOI: 10.1097/PRS.0b013e3182361f97

Abstract

BACKGROUND: In this study, the authors present a modification of the arteriovenous loop model that combines extrinsic and intrinsic vascularization modes to enhance vascularization of bioartificial matrices. METHODS: An arteriovenous loop was created in the medial thighs of 24 rats. The loop was placed in a newly developed titanium chamber, which was fabricated with an electron beam melting facility, and was embedded in a hydroxyapatite/β- tricalcium phosphate/fibrin matrix. At the explantation time points (2, 4, 6, and 8 weeks), constructs were perfused by differently colored dyes to determine the amount of tissue vascularized by either the intrinsic or the extrinsic vascular pathway. Specimens were investigated by means of micro-computed tomography and histologic and morphometric analysis. RESULTS: Although there was an equal number of blood vessels originating from the center and the periphery, 83 percent of all vessels displayed a connection to the arteriovenous loop already at 2 weeks. There was a continuous increase of the relative proportion of vessels connected to the arteriovenous loop over time detectable. At 8 weeks, communications between the newly formed vessels and the arteriovenous loop were visible in 97 percent of all vessels. CONCLUSIONS: This study demonstrates for the first time the enhancement of angiogenesis in an axially vascularized tissue by an additional extrinsic vascular pathway. By 2 weeks, both pathways showed connections, allowing transplantation of the entire construct using the arteriovenous loop pedicle. This approach will allow for reduction of the time interval between arteriovenous loop implantation and transplantation into the defect site and limitation of operative interventions. Copyright © 2011 by the American Society of Plastic Surgeons.

Authors with CRIS profile

Related research project(s)

Involved external institutions

Ruprecht-Karls-Universität Heidelberg Germany (DE)

How to cite

APA:

Arkudas, A., Galyna, P., Beier, J., Weigel, L., Körner, C., Singer, R.,... Kneser, U. (2012). Combination of extrinsic and intrinsic pathways significantly accelerates axial vascularization of bioartificial tissues. Plastic and Reconstructive Surgery, 129(1), 55e-65e. https://doi.org/10.1097/PRS.0b013e3182361f97

MLA:

Arkudas, Andreas, et al. "Combination of extrinsic and intrinsic pathways significantly accelerates axial vascularization of bioartificial tissues." Plastic and Reconstructive Surgery 129.1 (2012): 55e-65e.

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