Thymosin beta4 inhibits ADF/cofilin stimulated F-actin cycling and hela cell migration: Reversal by active Arp2/3 complex
Al Haj A, Mazur AJ, Buchmeier S, App C, Theiss C, Silvan U, Schoenenberger CA, Jockusch BM, Hannappel E, Weeds AG, Mannherz HG (2014)
Publication Type: Journal article
Publication year: 2014
Journal
Publisher: Wiley-Blackwell
Book Volume: 71
Pages Range: 95-107
Journal Issue: 2
DOI: 10.1002/cm.21128
Abstract
F-actin treadmilling plays a key part in cell locomotion. Because immunofluorescence showed colocalisation of thymosin beta4 (T?4) with cofilin-1 and Arp2/3 complex in lamellipodia, we analyzed combinations of these proteins on F-actin-adenosine triphosphate (ATP)-hydrolysis, which provides a measure of actin treadmilling. Actin depolymerising factor (ADF)/cofilin stimulated treadmilling, while T?4 decreased treadmilling, presumably by sequestering monomers. T?4 added together with ADF/cofilin also inhibited the treadmilling, relative to cofilin alone, but both the rate and extent of depolymerization were markedly enhanced in the presence of both these proteins. Arp2/3 complex reversed the sequestering activity of T?4 when equimolar to actin, but not in the additional presence of cofilin-1 or ADF. Transfection experiments to explore the effects of changing the intracellular concentration of T?4 in HeLa cells showed that an increase in T?4 resulted in reduced actin filaments bundles and narrower lamellipodia, and a conspicuous decrease of cell migration as seen by two different assays. In contrast, cells transfected with a vector leading to T?4 knockdown by small interfering RNA (siRNA) displayed prominent actin filament networks within the lamellipodia and the leading lamella and enhanced migration. The experiments reported here demonstrate the importance of the interplay of these different classes of actin-binding proteins on cell behaviour. © 2013 Wiley Periodicals, Inc.
Authors with CRIS profile
Christine App
Lehrstuhl für Biochemie und Pathobiochemie
Ewald Hannappel
Professur für Biochemie und Molekulare Neurowissenschaften
Involved external institutions
Ruhr-Universität Bochum (RUB)
Germany (DE)
Technische Universität Braunschweig
Germany (DE)
Universität Basel
Switzerland (CH)
Germany (DE)
Technische Universität Braunschweig
Germany (DE)
Universität Basel
Switzerland (CH)
How to cite
APA:
Al Haj, A., Mazur, A.J., Buchmeier, S., App, C., Theiss, C., Silvan, U.,... Mannherz, H.G. (2014). Thymosin beta4 inhibits ADF/cofilin stimulated F-actin cycling and hela cell migration: Reversal by active Arp2/3 complex. Cytoskeleton, 71(2), 95-107. https://doi.org/10.1002/cm.21128
MLA:
Al Haj, Abdulatif, et al. "Thymosin beta4 inhibits ADF/cofilin stimulated F-actin cycling and hela cell migration: Reversal by active Arp2/3 complex." Cytoskeleton 71.2 (2014): 95-107.
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