From Powder to Solution: Hydration Dependence of Human Hemoglobin Dynamics Correlated to Body Temperature
Stadler AM, Digel I, Embs JP, Unruh T, Tehei M, Zaccai G, Büldt G, Artmann G (2009)
Publication Status: Published
Publication Type: Journal article
Publication year: 2009
Journal
Publisher: CELL PRESS
Book Volume: 96
Pages Range: 5073-5081
Journal Issue: 12
DOI: 10.1016/j.bpj.2009.03.043
Abstract
A transition in hemoglobin (Hb), involving partial unfolding and aggregation, has been shown previously by various biophysical methods. The correlation between the transition temperature and body temperature for Hb from different species, suggested that it might be significant for biological function. To focus on such biologically relevant human Hb dynamics, we studied the protein internal picosecond motions as a response to hydration, by elastic and quasielastic neutron scattering. Rates of fast diffusive motions were found to be significantly enhanced with increasing hydration from fully hydrated powder to concentrated Hb solution. In concentrated protein solution, the data showed that amino acid side chains can explore larger volumes above body temperature than expected from normal temperature dependence. The body temperature transition in protein dynamics was absent in fully hydrated powder, indicating that picosecond protein dynamics responsible for the transition is activated only at a sufficient level of hydration. A collateral result from the study is that fully hydrated protein powder samples do not accurately describe all aspects of protein picosecond dynamics that might be necessary for biological function.
Authors with CRIS profile
Involved external institutions
Forschungszentrum Jülich / Research Centre Jülich (FZJ)
Germany (DE)
Fachhochschule Aachen - Aachen University of Applied Sciences
Germany (DE)
Paul Scherrer Institute (PSI)
Switzerland (CH)
University of Wollongong (UOW)
Australia (AU)
Institute Laue-Langevin (ILL)
France (FR)
Germany (DE)
Fachhochschule Aachen - Aachen University of Applied Sciences
Germany (DE)
Paul Scherrer Institute (PSI)
Switzerland (CH)
University of Wollongong (UOW)
Australia (AU)
Institute Laue-Langevin (ILL)
France (FR)
How to cite
APA:
Stadler, A.M., Digel, I., Embs, J.P., Unruh, T., Tehei, M., Zaccai, G.,... Artmann, G. (2009). From Powder to Solution: Hydration Dependence of Human Hemoglobin Dynamics Correlated to Body Temperature. Biophysical Journal, 96(12), 5073-5081. https://doi.org/10.1016/j.bpj.2009.03.043
MLA:
Stadler, Andreas M., et al. "From Powder to Solution: Hydration Dependence of Human Hemoglobin Dynamics Correlated to Body Temperature." Biophysical Journal 96.12 (2009): 5073-5081.
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