Sucrose transporter plays a role in phloem loading in CMV-infected melon plants that are defined as symplastic loaders

Gil L, Yaron I, Shalitin D, Sauer N, Turgeon R, Wolf S (2011)

Publication Language: English

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2011

Journal

Plant Journal Wiley

Book Volume: 66

Pages Range: 366-374

Journal Issue: 2

DOI: 10.1111/j.1365-313X.2011.04498.x

Open Access Link: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2011.04498.x/abstract

Abstract

Based on the high density of plasmodesmata interconnecting the intermediary cells and their neighboring phloem parenchyma or bundle-sheath cells, and based on the insensitivity to the sucrose transport inhibitor p- chloromercuribenzenesulfonic acid (PCMBS), cucurbits have been concluded to be symplastic loaders. In the present study, we identified and characterized the full-length sequence of sucrose transporter gene (CmSUT1) from melon (Cucumis melo L. cv. Hale's best jumbo). In vitro experiments confirmed that the identified gene product has sucrose transporter activity in baker's yeast. Healthy and cucumber mosaic virus (CMV)-infected melon plants were employed to examine sucrose transporter activity in planta. Pretreatment with PCMBS inhibited loading of newly fixed CO into minor veins of CMV-infected plants. Moreover, CMV infection caused significant increase in CmSUT1 transcripts expression, mainly in vascular bundles of minor veins, which was associated with elevated sucrose content in phloem sap collected from source-leaf petioles. We propose that cucurbit plants contain the machinery for apoplastic phloem loading and that CMV infection causes a quantitative shift in the mode by which photoassimilates are loaded into the sieve tube. © 2011 Blackwell Publishing Ltd.

Authors with CRIS profile

Norbert Sauer Lehrstuhl für Molekulare Pflanzenphysiologie

Involved external institutions

Hebrew University of Jerusalem IL Israel (IL) Cornell University US United States (USA) (US)

How to cite

APA:

Gil, L., Yaron, I., Shalitin, D., Sauer, N., Turgeon, R., & Wolf, S. (2011). Sucrose transporter plays a role in phloem loading in CMV-infected melon plants that are defined as symplastic loaders. Plant Journal, 66(2), 366-374. https://doi.org/10.1111/j.1365-313X.2011.04498.x

MLA:

Gil, Lidor, et al. "Sucrose transporter plays a role in phloem loading in CMV-infected melon plants that are defined as symplastic loaders." Plant Journal 66.2 (2011): 366-374.

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