Control of the receptor binding to a ligand-containing monolayer at the air/water interface by the lateral pressure: A film balance and neutron and infrared reflection study

Maierhofer A, Braun M, Vostrowsky O, Hirsch A, Langridge S, Bayerl T (2001)


Publication Type: Journal article, Original article

Publication year: 2001

Journal

Original Authors: Maierhofer A.P., Braun M., Vostrowsky O., Hirsch A., Langridge S., Bayerl T.M.

Publisher: American Chemical Society

Book Volume: 105

Pages Range: 3639-3645

Journal Issue: 17

URI: https://www.scopus.com/record/display.uri?eid=2-s2.0-0037013359&origin=inward

Abstract

We have studied the structure of a binary monolayer of dipalmitoylphosphatidylcholine (DPPC) and a biotinylated amphiphilic fullerene (BF) as a function of the lateral pressure. The compression isotherm of the mixture exhibited two separate kinks with a remarkably flat plateau after the second kink. Neutron and infrared reflection measurements suggest that upon compression of the mixed monolayer a (reversible) vertical shift of the BF occurs, leading to a (partial) demixing of the two components in the third dimension at high lateral pressure. As a consequence, the biotin group attached to the BF becomes inaccessible to streptavidin binding from the subphase at high lateral pressure, while the binding is clearly observed at low pressure. © 2001 American Chemical Society.

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How to cite

APA:

Maierhofer, A., Braun, M., Vostrowsky, O., Hirsch, A., Langridge, S., & Bayerl, T. (2001). Control of the receptor binding to a ligand-containing monolayer at the air/water interface by the lateral pressure: A film balance and neutron and infrared reflection study. Journal of Physical Chemistry B, 105(17), 3639-3645.

MLA:

Maierhofer, Andreas, et al. "Control of the receptor binding to a ligand-containing monolayer at the air/water interface by the lateral pressure: A film balance and neutron and infrared reflection study." Journal of Physical Chemistry B 105.17 (2001): 3639-3645.

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