Validation of the reliability of computational O-GlcNAc prediction

Jochmann R, Holz P, Sticht H, Stürzl M (2014)

Publication Type: Journal article

Publication year: 2014


Publisher: Elsevier

Book Volume: 1844

Pages Range: 416-21

Journal Issue: 2

DOI: 10.1016/j.bbapap.2013.12.002


O-GlcNAcylation is an inducible, highly dynamic and reversible posttranslational modification, which regulates numerous cellular processes such as gene expression, translation, immune reactions, protein degradation, protein-protein interaction, apoptosis, and signal transduction. In contrast to N-linked glycosylation, O-GlcNAcylation does not display a strict amino acid consensus sequence, although serine or threonine residues flanked by proline and valine are preferred sites of O-GlcNAcylation. Based on this information, computational prediction tools of O-GlcNAc sites have been developed. Here, we retrospectively assessed the performance of two available O-GlcNAc prediction programs YinOYang 1.2 server and OGlcNAcScan by comparing their predictions for recently discovered experimentally validated O-GlcNAc sites. Both prediction programs efficiently identified O-GlcNAc sites situated in an environment resembling the consensus sequence P-P-V-[ST]-T-A. However, both prediction programs revealed numerous false negative O-GlcNAc predictions when the site of modification was located in an amino acid sequence differing from the known consensus sequence. By searching for a common sequence motif, we found that O-GlcNAcylation of nucleocytoplasmic proteins preferably occurs at serine and threonine residues flanked downstream by proline and valine and upstream by one to two alanines followed by a stretch of serine and threonine residues. However, O-GlcNAcylation of proteins located in the mitochondria or in the secretory lumen occurs at different sites and does not follow a distinct consensus sequence. Thus, our study indicates the limitations of the presently available computational prediction methods for O-GlcNAc sites and suggests that experimental validation is mandatory. Continuously update and further development of available databases will be the key to improve the performance of O-GlcNAc site prediction.

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Jochmann, R., Holz, P., Sticht, H., & Stürzl, M. (2014). Validation of the reliability of computational O-GlcNAc prediction. Biochimica Et Biophysica Acta-Proteins and Proteomics, 1844(2), 416-21.


Jochmann, Ramona, et al. "Validation of the reliability of computational O-GlcNAc prediction." Biochimica Et Biophysica Acta-Proteins and Proteomics 1844.2 (2014): 416-21.

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