Reliable quantification of 1,2-dihydroxynaphthalene in urine using a conjugated reference compound for calibration
Zobel M, Göen T, Belov V, Klotz K (2017)
Publication Type: Journal article
Publication year: 2017
Journal
Book Volume: 409
Pages Range: 6861-6872
Journal Issue: 29
DOI: 10.1007/s00216-017-0651-9
Abstract
After environmental and occupational exposure to naphthalene, 1,2-dihydroxynaphthalene (1,2-DHN) was shown to be one major metabolite in human naphthalene metabolism. However, the instability of free 1,2-DHN complicates the reliable determination of this promising biomarker in urine. To solve this stability problem, glucuronide conjugates of 1,2-DHN and the corresponding isotopically labelled D6-1,2-dihydroxynaphthalene (D6-1,2-DHN) were synthesised and applied as reference material and internal standard in a gas chromatographic-tandem mass spectrometric (GC-MS/MS) method. The determination of 1- and 2-naphthol (1-MHN, 2-MHN) was included in the procedure to enable a comprehensive assessment of naphthalene metabolism and exposure. The results of the validation showed a high reliability and sensitivity of the method. The detection limits range from 0.05 to 0.16 ?g/L. Precision and repeatability were determined to range from 1.4 to 6.6% for all parameters. The simultaneous determination of 1- and 2-MHN as additional parameters besides 1,2-DHN enables the application of the method for further metabolism and kinetic studies on naphthalene. The use of glucuronide-derivative reference substances and the application of structurally matched isotopic-labelled internal standards for each substance guarantee a reliable quantification of the main naphthalene metabolites 1,2-DHN and 1- and 2-MHN. Graphical abstract Reliable quantification of 1,2-dihydroxynaphthalene in urine using a conjugated reference compound for calibration.
Authors with CRIS profile
Melanie Zobel
Lehrstuhl für Arbeits-, Sozial- und Umweltmedizin
Thomas Göen
Medizinische Fakultät
Katrin Klotz
Lehrstuhl für Arbeits-, Sozial- und Umweltmedizin
Involved external institutions
Germany (DE)How to cite
APA:
Zobel, M., Göen, T., Belov, V., & Klotz, K. (2017). Reliable quantification of 1,2-dihydroxynaphthalene in urine using a conjugated reference compound for calibration. Analytical and Bioanalytical Chemistry, 409(29), 6861-6872. https://doi.org/10.1007/s00216-017-0651-9
MLA:
Zobel, Melanie, et al. "Reliable quantification of 1,2-dihydroxynaphthalene in urine using a conjugated reference compound for calibration." Analytical and Bioanalytical Chemistry 409.29 (2017): 6861-6872.
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