Bovine mastitis associated with Prototheca blaschkeae

Marques S, Silva E, Kraft C, Carvalheira J, Videira A, Huß V, Thompson G (2008)

Publication Language: English

Publication Type: Journal article, Original article

Publication year: 2008

Journal

Journal of Clinical Microbiology American Society for Microbiology

Book Volume: 46

Pages Range: 1941–1945

Journal Issue: 6

DOI: 10.1128/JCM.00323-08

Abstract

Bovine mastitis is an important and complex disease responsible for economic losses in the dairy industry.
Biotype II strains of the green alga Prototheca zopfii can be involved, most often resulting in chronic mastitis
of difficult treatment associated with reduced milk production. This type of infection is rare, but the number
of reported cases is increasing worldwide. In order to determine the kind of species involved in mastitis by
Prototheca in northwest Portugal, 41 Prototheca isolates were genetically characterized. The algae are part of
Prototheca isolates that were collected during a 6-year period, isolated from the milk of 41 dairy cows in a total
of 22 herds with a history of increasing somatic cell counts, mild clinical signs of udder infection, and
unsuccessful response to the usual therapy. PCR amplification of the 18S ribosomal DNA (rDNA), amplified
rDNA restriction analysis, and phylogenetic analyses of the 18S rDNA sequences were performed. Thirty-seven
isolates were identified as P. zopfii var. hydrocarbonea and four as Prototheca blaschkeae. These data suggest a
high incidence of P. zopfii var. hydrocarbonea mastitis in the region and demonstrate for the first time the
involvement of P. blaschkeae with bovine mammary gland infections.

Authors with CRIS profile

Volker Huß Lehrstuhl für Molekulare Pflanzenphysiologie

Involved external institutions

Research Centre in Biodiversity and Genetic Resources PT Portugal (PT)

How to cite

APA:

Marques, S., Silva, E., Kraft, C., Carvalheira, J., Videira, A., Huß, V., & Thompson, G. (2008). Bovine mastitis associated with Prototheca blaschkeae. Journal of Clinical Microbiology, 46(6), 1941–1945. https://doi.org/10.1128/JCM.00323-08

MLA:

Marques, Sara, et al. "Bovine mastitis associated with Prototheca blaschkeae." Journal of Clinical Microbiology 46.6 (2008): 1941–1945.

BibTeX: Download