Rational design of ?-sheet ligands against A?42-induced toxicity

Hochdoerffer K, Maerz-Berberich J, Nage-Steger L, Epple M, Meyer-Zaika W, Horn A, Sticht H, Sinha S, Bitan G, Schrader T (2011)

Publication Type: Journal article

Publication year: 2011


Book Volume: 133

Pages Range: 4348-58

Journal Issue: 12

DOI: 10.1021/ja107675n


A ?-sheet-binding scaffold was equipped with long-range chemical groups for tertiary contacts toward specific regions of the Alzheimer's A? fibril. The new constructs contain a trimeric aminopyrazole carboxylic acid, elongated with a C-terminal binding site, whose influence on the aggregation behavior of the A?(42) peptide was studied. MD simulations after trimer docking to the anchor point (F19/F20) suggest distinct groups of complex structures each of which featured additional specific interactions with characteristic A? regions. Members of each group also displayed a characteristic pattern in their antiaggregational behavior toward A?. Specifically, remote lipophilic moieties such as a dodecyl, cyclohexyl, or LPFFD fragment can form dispersive interactions with the nonpolar cluster of amino acids between I31 and V36. They were shown to strongly reduce Thioflavine T (ThT) fluorescence and protect cells from A? lesions (MTT viability assays). Surprisingly, very thick fibrils and a high ?-sheet content were detected in transmission electron microscopy (TEM) and CD spectroscopic experiments. On the other hand, distant single or multiple lysines which interact with the ladder of stacked E22 residues found in A? fibrils completely dissolve existing ?-sheets (ThT, CD) and lead to unstructured, nontoxic material (TEM, MTT). Finally, the triethyleneglycol spacer between heterocyclic ?-sheet ligand and appendix was found to play an active role in destabilizing the turn of the U-shaped protofilament. Fluorescence correlation spectroscopy (FCS) and sedimentation velocity analysis (SVA) provided experimental evidence for some smaller benign aggregates of very thin, delicate structure (TEM, MTT). A detailed investigation by dynamic light scattering (DLS) and other methods proved that none of the new ligands acts as a colloid. The evolving picture for the disaggregation mechanism by these new hybrid ligands implies transformation of well-ordered fibrils into less structured aggregates with a high molecular weight. In the few cases where fibrillar components remain, these display a significantly altered morphology and have lost their acute cellular toxicity.

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Hochdoerffer, K., Maerz-Berberich, J., Nage-Steger, L., Epple, M., Meyer-Zaika, W., Horn, A.,... Schrader, T. (2011). Rational design of ?-sheet ligands against A?42-induced toxicity. Journal of the American Chemical Society, 133(12), 4348-58. https://doi.org/10.1021/ja107675n


Hochdoerffer, Katrin, et al. "Rational design of ?-sheet ligands against A?42-induced toxicity." Journal of the American Chemical Society 133.12 (2011): 4348-58.

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