Influence of Different Antioxidants on X-Ray Induced DNA Double-Strand Breaks (DSBs) Using ?-H2AX Immunofluorescence Microscopy in a Preliminary Study

Brand M, Sommer M, Ellmann S, Wüst W, May M, Eller A, Vogt S, Lell M, Kuefner MA, Uder M (2015)

Publication Type: Journal article

Publication year: 2015

Journal

PLoS ONE Public Library of Science

Book Volume: 10

Pages Range: e0127142

Journal Issue: 5

DOI: 10.1371/journal.pone.0127142

Abstract

Radiation exposure occurs in X-ray guided interventional procedures or computed tomography (CT) and ?-H2AX-foci are recognized to represent DNA double-strand breaks (DSBs) as a biomarker for radiation induced damage. Antioxidants may reduce the induction of ?-H2AX-foci by binding free radicals. The aim of this study was to establish a dose-effect relationship and a time-effect relationship for the individual antioxidants on DSBs in human blood lymphocytes.Blood samples from volunteers were irradiated with 10 mGy before and after pre-incubation with different antioxidants (zinc, trolox, lipoic acid, ß-carotene, selenium, vitamin E, vitamin C, N-acetyl-L-cysteine (NAC) and Q 10). Thereby, different pre-incubation times, concentrations and combinations of drugs were evaluated. For assessment of DSBs, lymphocytes were stained against the phosphorylated histone variant ?-H2AX.For zinc, trolox and lipoic acid regardless of concentration or pre-incubation time, no significant decrease of ?-H2AX-foci was found. However, ß-carotene (15%), selenium (14%), vitamin E (12%), vitamin C (25%), NAC (43%) and Q 10 (18%) led to a significant reduction of ?-H2AX-foci at a pre-incubation time of 1 hour. The combination of different antioxidants did not have an additive effect.Antioxidants administered prior to irradiation demonstrated the potential to reduce ?-H2AX-foci in blood lymphocytes.

Authors with CRIS profile

Michael Brand Lehrstuhl für Diagnostische Radiologie Matthias Sommer Institute of Radiology Stephan Ellmann Institute of Radiology Wolfgang Wüst Medizinische Fakultät Matthias May Institute of Radiology Sabine Vogt Medizinische Fakultät Michael Lell Medizinische Fakultät Michael Uder Lehrstuhl für Diagnostische Radiologie

How to cite

APA:

Brand, M., Sommer, M., Ellmann, S., Wüst, W., May, M., Eller, A.,... Uder, M. (2015). Influence of Different Antioxidants on X-Ray Induced DNA Double-Strand Breaks (DSBs) Using ?-H2AX Immunofluorescence Microscopy in a Preliminary Study. PLoS ONE, 10(5), e0127142. https://doi.org/10.1371/journal.pone.0127142

MLA:

Brand, Michael, et al. "Influence of Different Antioxidants on X-Ray Induced DNA Double-Strand Breaks (DSBs) Using ?-H2AX Immunofluorescence Microscopy in a Preliminary Study." PLoS ONE 10.5 (2015): e0127142.

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