Tetracycline sensing using novel doxycycline derivatives immobilized on different surface plasmon resonance biosensor surfaces
Andree U, Müller-Seitz E, Usai I, Löber S, Gmeiner P, Wimmer C, Hillen W, Petz M (2010)
Publication Type: Journal article, Original article
Publication year: 2010
Journal
Original Authors: Andree U., Mueller-Seitz E., Usai I., Loeber S., Gmeiner P., Wimmer C., Hillen W., Petz M.
Publisher: Future Science
Book Volume: 2
Pages Range: 217-227
Journal Issue: 2
DOI: 10.4155/bio.09.176
Abstract
Background: This article aims to explore novel doxycycline derivatives for analyzing low concentrations of tetracyclines in biological matrices and food in competitive assays. Results: Surface plasmon resonance (SPR) was employed in an indirect competitive format using a bacterial tetracycline-dependent regulatory protein as receptor. Three doxycycline derivatives were synthesized and covalently bound to the surface of four different sensor chips. Parameters that influence the immobilization of the doxycycline derivatives and subsequent binding of the receptor protein were studied. Conclusion: The novel doxycycline derivatives were successfully used as competitors in an indirect SPR assay. © 2010 Future Science Ltd.
Authors with CRIS profile
Stefan Löber
Lehrstuhl für Pharmazeutische Chemie
Peter Gmeiner
Lehrstuhl für Pharmazeutische Chemie
Cornelius Wimmer
Professur für Mikrobiologie
Involved external institutions
Germany (DE)How to cite
APA:
Andree, U., Müller-Seitz, E., Usai, I., Löber, S., Gmeiner, P., Wimmer, C.,... Petz, M. (2010). Tetracycline sensing using novel doxycycline derivatives immobilized on different surface plasmon resonance biosensor surfaces. Bioanalysis, 2(2), 217-227. https://doi.org/10.4155/bio.09.176
MLA:
Andree, Ulrike, et al. "Tetracycline sensing using novel doxycycline derivatives immobilized on different surface plasmon resonance biosensor surfaces." Bioanalysis 2.2 (2010): 217-227.
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