Formalin evokes calcium transients from the endoplasmatic reticulum
Fischer M, Soller KJ, Sauer S, Kalucka J, Veglia G, Reeh P (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 10
Pages Range: e0123762
Journal Issue: 4
DOI: 10.1371/journal.pone.0123762
Abstract
The formalin test is the most widely used behavioral screening test for analgesic compounds. The cellular mechanism of action of formaldehyde, inducing a typically biphasic pain-related behavior in rodents is addressed in this study. The chemoreceptor channel TRPA1 was suggested as primary transducer, but the high concentrations used in the formalin test elicit a similar response in TRPA1 wildtype and knockout animals. Here we show that formaldehyde evokes a dose-dependent calcium release from intracellular stores in mouse sensory neurons and primary keratinocytes as well as in non-neuronal cell lines, and independent of TRPA1. The source of calcium is the endoplasmatic reticulum and inhibition of the sarco/endoplasmic reticulum calcium-ATPase has a major contribution. This TRPA1-independent mechanism may underlie formaldehyde-induced pan-neuronal excitation and subsequent inflammation.
Authors with CRIS profile
Michael Fischer
Medizinische Fakultät
Susanne Sauer
Lehrstuhl für Physiologie
Peter Reeh
Professur für Physiologie
Involved external institutions
University of Minnesota (UMN)
United States (USA) (US)
Katholieke Universiteit Leuven (KUL) / Catholic University of Leuven
Belgium (BE)
United States (USA) (US)
Katholieke Universiteit Leuven (KUL) / Catholic University of Leuven
Belgium (BE)
How to cite
APA:
Fischer, M., Soller, K.J., Sauer, S., Kalucka, J., Veglia, G., & Reeh, P. (2015). Formalin evokes calcium transients from the endoplasmatic reticulum. PLoS ONE, 10(4), e0123762. https://doi.org/10.1371/journal.pone.0123762
MLA:
Fischer, Michael, et al. "Formalin evokes calcium transients from the endoplasmatic reticulum." PLoS ONE 10.4 (2015): e0123762.
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