Human lymphoid organ dendritic cell identity is predominantly dictated by ontogeny, not tissue microenvironment

Heidkamp GF, Sander J, Lehmann C, Heger L, Eissing N, Baranska A, Lühr J, Hoffmann A, Reimer K, Lux A, Söder S, Hartmann A, Zenk J, Ulas T, Mcgovern N, Alexiou C, Spriewald B, Mackensen A, Schuler G, Schauf B, Forster A, Repp R, Fasching P, Purbojo A, Cesnjevar R, Ullrich E, Ginhoux F, Schlitzer A, Nimmerjahn F, Schultze JL, Dudziak D (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Science immunology American Association for the Advancement of Science

Book Volume: 1

Journal Issue: 6

DOI: 10.1126/sciimmunol.aai7677

Abstract

In mice, conventional and plasmacytoid dendritic cells (DCs) derive from separate hematopoietic precursors before they migrate to peripheral tissues. Moreover, two classes of conventional DCs (cDC1 and cDC2 DCs) and one class of plasmacytoid DCs (pDCs) have been shown to be transcriptionally and functionally distinct entities. In humans, these three DC subtypes can be identified using the cell surface markers CD1c (cDC2), CD141 (cDC1), and CD303 (pDCs), albeit it remains elusive whether DC functionality is mainly determined by ontogeny or the tissue microenvironment. By phenotypic and transcriptional profiling of these three DC subtypes in different human tissues derived from a large number of human individuals, we demonstrate that DC subpopulations in organs of the lymphohematopoietic system (spleen, thymus, and blood) are strongly defined by ontogeny rather than by signals from the microenvironment. In contrast, DC subsets derived from human lung or skin differed substantially, strongly arguing that DCs react toward modulatory signals from tissue microenvironments. Collectively, the data obtained in this study may serve as a major resource to guide further studies into human DC biology during homeostasis and inflammation.

Authors with CRIS profile

Gordon Frederik Heidkamp Department of Dermatology Christian Lehmann Department of Dermatology Lukas Heger Department of Dermatology Jennifer Lühr Department of Obstetrics and Gynaecology Alana Hoffmann Department of Molecular Neurology Katharina Reimer Professur für die Biologie Dendritischer Zellen Anja Lux Lehrstuhl für Genetik Arndt Hartmann Lehrstuhl für Allgemeine Pathologie und Pathologische Anatomie Christoph Alexiou Professur für Nanomedizin Bernd Spriewald Medizinische Fakultät Andreas Mackensen Lehrstuhl für Innere Medizin V Gerold Schuler Lehrstuhl für Haut- und Geschlechtskrankheiten Peter Fasching Professur für Translationale Frauenheilkunde und Geburtshilfe Ariawan Purbojo Department of Paediatric Cardiac Surgery Robert Cesnjevar Department of Paediatric Cardiac Surgery Falk Nimmerjahn Lehrstuhl für Genetik Diana Dudziak Professur für die Biologie Dendritischer Zellen

Additional Organisation(s)

Interdisziplinäres Zentrum für Klinische Forschung

Involved external institutions

Sozialstiftung Bamberg DE Germany (DE) Agency for Science, Technology and Research (A*STAR) SG Singapore (SG) Rheinische Friedrich-Wilhelms-Universität Bonn DE Germany (DE) Städtisches Krankenhaus Kiel DE Germany (DE) Goethe-Universität Frankfurt am Main DE Germany (DE) Klinikum Augsburg DE Germany (DE)

How to cite

APA:

Heidkamp, G.F., Sander, J., Lehmann, C., Heger, L., Eissing, N., Baranska, A.,... Dudziak, D. (2016). Human lymphoid organ dendritic cell identity is predominantly dictated by ontogeny, not tissue microenvironment. Science immunology, 1(6). https://doi.org/10.1126/sciimmunol.aai7677

MLA:

Heidkamp, Gordon Frederik, et al. "Human lymphoid organ dendritic cell identity is predominantly dictated by ontogeny, not tissue microenvironment." Science immunology 1.6 (2016).

BibTeX: Download