A transposon-based activation-tagging population in Arabidopsis thaliana (TAMARA) and its application in the identification of dominant developmental and metabolic mutations

Sonnewald U (2005)


Publication Status: Published

Publication Type: Journal article

Publication year: 2005

Journal

Publisher: ELSEVIER SCIENCE BV

Book Volume: 579

Pages Range: 4622-4628

Journal Issue: 21

DOI: 10.1016/j.febslet.2005.07.030

Abstract

A population of 9471 stable activation-tagged lines was generated by transposable element mediated activation tagging mutagenesis in Arabidopsis (TAMARA) using the maize EnlSpm transposon system. Based on DNA gel blot and flanking sequence analysis, this population contains approximately 6000 independent transposon insertions. A greenhouse-based screen identified six dominant or semi-dominant activation tagged mutants with obvious developmental alterations, among these a new pistillata mutant allele. In addition, a subset of 1500 lines was screened by a HPLC based high-throughput method for dominant activation tagged mutants with enhanced contents of phenolic compounds. One dominant activation tagged mutant (hpc1-1D) was isolated showing accumulation of a particular compound due to the upregulation of an R2R3-MYB transcription factor. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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How to cite

APA:

Sonnewald, U. (2005). A transposon-based activation-tagging population in Arabidopsis thaliana (TAMARA) and its application in the identification of dominant developmental and metabolic mutations. Febs Letters, 579(21), 4622-4628. https://doi.org/10.1016/j.febslet.2005.07.030

MLA:

Sonnewald, Uwe. "A transposon-based activation-tagging population in Arabidopsis thaliana (TAMARA) and its application in the identification of dominant developmental and metabolic mutations." Febs Letters 579.21 (2005): 4622-4628.

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