Antagonist-induced deadhesion of specifically adhered vesicles
Smith AS, Lorz BG, Seifert U, Sackmann E (2006)
Publication Language: English
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 2006
Journal
Publisher: CELL PRESS
Book Volume: 90
Pages Range: 1064-1080
Journal Issue: 3
URI: https://www.ncbi.nlm.nih.gov/pubmed/16272442
DOI: 10.1529/biophysj.105.062166
Abstract
By use of a model system consisting of giant vesicles adhering to. at substrates, we identified, both experimentally and theoretically, two new control mechanisms for antagonist-induced deadhesion. Adhesion is established by specific binding of surface-grafted E-selectin and vesicle-carrying oligosaccharide Lewis(X). Deadhesion is achieved by controlled titration of monoclonal antibodies against E-selectin. The first mechanism is characterized by a considerable retraction of the contact zone resulting in a loss of contact area between the vesicle and the substrate. Within the developed theoretical framework, the observed equilibrium state is understood as a balance between the spreading pressure of the vesicle and the antagonist-induced lateral pressure at the edge of the contact zone. In the second mechanism, the antibodies induce unbinding by penetrating the contact zone without significantly affecting its size. This process reveals the decomposition of the adhesion zone into microdomains of tight binding separated by strongly fluctuating sections of the membrane. Both experiment and theory show a sigmoidal decrease of the number of bound ligands as a function of the logarithm of antagonist concentration. The work presented herein also provides a new method for the determination of the receptor binding affinity of either the surface-embedded ligands or the competing antagonist molecules.
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APA:
Smith, A.-S., Lorz, B.G., Seifert, U., & Sackmann, E. (2006). Antagonist-induced deadhesion of specifically adhered vesicles. Biophysical Journal, 90(3), 1064-1080. https://doi.org/10.1529/biophysj.105.062166
MLA:
Smith, Ana-Suncana, et al. "Antagonist-induced deadhesion of specifically adhered vesicles." Biophysical Journal 90.3 (2006): 1064-1080.
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