Eckert E, Göen T (2014)
Publication Type: Journal article
Publication year: 2014
Publisher: Elsevier
Book Volume: 965
Pages Range: 54-60
DOI: 10.1016/j.jchromb.2014.06.009
We developed and validated an analytical method for the simultaneous determination of methyl mercapturic acid (MeMA), ethyl mercapturic acid (EtMA), n-propyl mercapturic acid (PrMA) and iso-propyl mercapturic acid (iPrMA) in human urine. These alkyl mercapturic acids are known or presumed biomarkers of exposure to several alkylating agents including methyl bromide, dimethyl sulfate, ethyl bromide, 1-bromopropane and 2-bromopropane. The method involves a column switching arrangement for online solid phase extraction of the analytes with subsequent analytical separation and detection using liquid chromatography and tandem mass spectrometry. Within day and day-to-day imprecision was determined to range from 4.5 to 12.2%. The analytical method is distinguished by its wide linear working range of up to 2,500 ?g/L with detection limits ranging from 2.0 ?g/L (for PrMA) to 5.1 ?g/L (for MeMA) that render possible the application in various biomonitoring studies regarding exposure to alkylating agents. The results of a pilot study on urine samples of 30 individuals occupationally non-exposed to alkylating agents using the new procedure confirmed the background excretion of MeMA (<5.1-35.6 ?g/L) and PrMA (<2.0-95.7 ?g/L).
APA:
Eckert, E., & Göen, T. (2014). Rapid determination of four short-chain alkyl mercapturic acids in human urine by column-switching liquid chromatography-tandem mass spectrometry. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 965, 54-60. https://doi.org/10.1016/j.jchromb.2014.06.009
MLA:
Eckert, Elisabeth, and Thomas Göen. "Rapid determination of four short-chain alkyl mercapturic acids in human urine by column-switching liquid chromatography-tandem mass spectrometry." Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences 965 (2014): 54-60.
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