SFTA3, a novel protein of the lung: three-dimensional structure, characterisation and immune activation

Schicht M, Rausch F, Finotto S, Mathews M, Mattil A, Schubert M, Koch B, Traxdorf M, Bohr C, Worlitzsch D, Brandt W, Garreis F, Sel S, Paulsen F, Bräuer L (2014)

Publication Type: Journal article

Publication year: 2014

Journal

European Respiratory Journal European Respiratory Society

Publisher: European Respiratory Society: ERJ

Book Volume: 44

Pages Range: 447-56

Journal Issue: 2

DOI: 10.1183/09031936.00179813

Abstract

The lung constantly interacts with numerous pathogens. Thus, complex local immune defence mechanisms are essential to recognise and dispose of these intruders. This work describes the detection, characterisation and three-dimensional structure of a novel protein of the lung (surfactant-associated protein 3 (SFTA3/SP-H)) with putative immunological features. Bioinformatics, biochemical and immunological methods were combined to elucidate the structure and function of SFTA3. The tissue-specific detection and characterisation was performed by using electron microscopy as well as fluorescence imaging. Three-dimensional structure generation and analysis led to the development of specific antibodies and, as a consequence, to the localisation of a novel protein in human lung under consideration of cystic fibrosis, asthma and sepsis. In vitro experiments revealed that lipopolysaccharide induces expression of SFTA3 in the human lung alveolar type II cell line A549. By contrast, the inflammatory cytokines interleukin (IL)-1? and IL-23 inhibit expression of SFTA3 in A549. Sequence- and structure-based prediction analysis indicated that the novel protein is likely to belong to the family of lung surfactant proteins. The results suggest that SFTA3 is an immunoregulatory protein of the lung with relevant protective functions during inflammation at the mucosal sites.

Authors with CRIS profile

Martin Schicht Lehrstuhl für Funktionelle und Klinische Anatomie Felix Rausch Lehrstuhl für Funktionelle und Klinische Anatomie Susetta Neurath-Finotto Department of Molecular Pneumology Christopher Bohr Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik) Fabian Garreis Lehrstuhl für Funktionelle und Klinische Anatomie Friedrich Paulsen Lehrstuhl für Funktionelle und Klinische Anatomie Lars Bräuer Professur für Oberflächenaktive Proteine

Involved external institutions

Martin-Luther-Universität Halle-Wittenberg (MLU) DE Germany (DE) Leibniz Institute of Plant Biochemistry / Leibniz-Institut für Pflanzenbiochemie DE Germany (DE) Ruprecht-Karls-Universität Heidelberg DE Germany (DE)

How to cite

APA:

Schicht, M., Rausch, F., Finotto, S., Mathews, M., Mattil, A., Schubert, M.,... Bräuer, L. (2014). SFTA3, a novel protein of the lung: three-dimensional structure, characterisation and immune activation. European Respiratory Journal, 44(2), 447-56. https://doi.org/10.1183/09031936.00179813

MLA:

Schicht, Martin, et al. "SFTA3, a novel protein of the lung: three-dimensional structure, characterisation and immune activation." European Respiratory Journal 44.2 (2014): 447-56.

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