Polisetti N, Zenkel M, Menzel-Severing J, Kruse F, Schlötzer-Schrehardt U (2016)
Publication Type: Journal article
Publication year: 2016
Book Volume: 34
Pages Range: 203-19
Journal Issue: 1
DOI: 10.1002/stem.2191
Interactions between stem cells and their microenvironment are critical for regulation and maintenance of stem cell function. To elucidate the molecular interactions within the human limbal epithelial stem/progenitor cell (LEPC) niche, which is essential for maintaining corneal transparency and vision, we performed a comprehensive expression analysis of cell adhesion molecules (CAMs) using custom-made quantitative real-time polymerase chain reaction (qRT-PCR) arrays and laser capture-microdissected LEPC clusters, comprising LEPCs, melanocytes, mesenchymal cells, and transmigrating immune cells. We show that LEPCs are anchored to their supporting basement membrane by the laminin receptors ?3?1 and ?6?4 integrin and the dystroglycan complex, while intercellular contacts between LEPCs and melanocytes are mediated by N-, P-, and E-cadherin together with L1-CAM, a member of the immunoglobulin superfamily (Ig)CAMs. In addition to the LEPC-associated heparan sulfate proteoglycans syndecan-2, glypican-3, and glypican-4, the IgCAM members ICAM-1 and VCAM-1 were found to be variably expressed on LEPCs and associated niche cells and to be dynamically regulated in response to chemokines such as interferon-? to enhance interactions with immune cells. Moreover, junctional adhesion molecule JAM-C accumulating in the subepithelial limbal matrix, appeared to be involved in recruitment of immune cells, while mesenchymal stromal cells appeared to use the nephronectin receptor integrin ?8 for approaching the limbal basement membrane. In summary, we identified a novel combination of cell surface receptors that may regulate both stable and dynamic cell-matrix and cell-cell interactions within the limbal niche. The findings provide a solid foundation for further functional studies and for advancement of our current therapeutic strategies for ocular surface reconstruction. Stem Cells 2016;34:203-219.
APA:
Polisetti, N., Zenkel, M., Menzel-Severing, J., Kruse, F., & Schlötzer-Schrehardt, U. (2016). Cell Adhesion Molecules and Stem Cell-Niche-Interactions in the Limbal Stem Cell Niche. Stem Cells, 34(1), 203-19. https://doi.org/10.1002/stem.2191
MLA:
Polisetti, Naresh, et al. "Cell Adhesion Molecules and Stem Cell-Niche-Interactions in the Limbal Stem Cell Niche." Stem Cells 34.1 (2016): 203-19.
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