Recovering the superficial microvascular pattern via diffuse reflection imaging: phantom validation

Chen C, Klämpfl F, Kanawade R, Riemann M, Knipfer C, Stelzle F, Schmidt M (2015)

Publication Language: English

Publication Type: Journal article, Review article

Publication year: 2015

Journal

Biomedical Engineering Online BioMed Central

Publisher: BioMed Central

Book Volume: 14

Pages Range: 87 - 101

Journal Issue: 1

DOI: 10.1186/s12938-015-0081-x

Open Access Link: http://www.biomedical-engineering-online.com/content/14/1/87/abstract

Abstract

Background

Diffuse reflection imaging could potentially be used to recover the superficial microvasculature under cutaneous tissue and the associated blood oxygenation status with a modified imaging resolution. The aim of this work is to deliver a new approach of local off-axis scanning diffuse reflection imaging, with the revisit of the modified Beer–Lambert Law (MBLL).

Methods

To validate this, the system is used to recover the micron-scale subsurface vessel structure interiorly embedded in a skin equivalent tissue phantom. This vessel structure is perfused with oxygenated meta-hemoglobin solution.

Results

Our preliminary results confirm that the thin vessel structure can be mapped into a 2-D planar image. The distributions of oxygenated hemoglobin concentration (<a onClick="popup('http://www.biomedical-engineering-online.com/content/14/1/87/mathml/M1','MathML',630,470);return false;" target="_blank" data-cke-saved-href="http://www.biomedical-engineering-online.com/content/14/1/87/mathml/M1" href="http://www.biomedical-engineering-online.com/content/14/1/87/mathml/M1">View MathML</a>) and deoxygenated hemoglobin concentration (<a onClick="popup('http://www.biomedical-engineering-online.com/content/14/1/87/mathml/M2','MathML',630,470);return false;" target="_blank" data-cke-saved-href="http://www.biomedical-engineering-online.com/content/14/1/87/mathml/M2" href="http://www.biomedical-engineering-online.com/content/14/1/87/mathml/M2">View MathML</a>) can be co-registerated through the MBLL upon the CW spectroscopy, the scattering issue is addressed in the reformed MBLL. The recovered pattern matches to the estimation from the simultaneous optical coherence tomography studies.

Conclusions

With further modification, this system may serve as the first prototype to investigate the superficial microvasculature in the expotential skin cancer loci, or a micro-lesion of vascular dermatosis.

Authors with CRIS profile

Chen Chen Lehrstuhl für Photonische Technologien (LPT) Florian Klämpfl Lehrstuhl für Photonische Technologien (LPT) Rajesh Kanawade Lehrstuhl für Photonische Technologien (LPT) Max Riemann Medizinische Fakultät Florian Stelzle Department of Oral and Cranio-Maxillofacial Surgery Michael Schmidt Lehrstuhl für Photonische Technologien (LPT)

How to cite

APA:

Chen, C., Klämpfl, F., Kanawade, R., Riemann, M., Knipfer, C., Stelzle, F., & Schmidt, M. (2015). Recovering the superficial microvascular pattern via diffuse reflection imaging: phantom validation. Biomedical Engineering Online, 14(1), 87 - 101. https://doi.org/10.1186/s12938-015-0081-x

MLA:

Chen, Chen, et al. "Recovering the superficial microvascular pattern via diffuse reflection imaging: phantom validation." Biomedical Engineering Online 14.1 (2015): 87 - 101.

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