Sonnewald U (2007)
Publication Status: Published
Publication Type: Journal article
Publication year: 2007
Publisher: ELSEVIER SCIENCE BV
Book Volume: 581
Pages Range: 2401-2410
Journal Issue: 13
DOI: 10.1016/j.febslet.2007.04.060
Plants possess two different types of phosphofructokinases, an ATP-dependent (PFK) and a pyrophosphate-dependent form (PFP). While plant PFPs have been investigated in detail, cDNA clones coding for PFK have not been identified in Arabidopsis thaliana. Searching the A. thaliana genome revealed 11 putative members of a phosphofructokinase gene family. Among those, four sequences showed high homology to the alpha- or beta-subunits of plant PFPs. Seven cDNAs resulted in elevated PFK, but not PFP activity after transient expression in tobacco leaves suggesting that they encode Arabidopsis PFKs. RT-PCR revealed different tissue-specific expression of the individual forms. Furthermore, analysis of GFP fusion proteins indicated their presence in different sub-cellular compartments. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
APA:
Sonnewald, U. (2007). Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana. Febs Letters, 581(13), 2401-2410. https://doi.org/10.1016/j.febslet.2007.04.060
MLA:
Sonnewald, Uwe. "Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana." Febs Letters 581.13 (2007): 2401-2410.
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