Virtual Hematoxylin and Eosin Transillumination Microscopy Using Epi-Fluorescence Imaging
Giacomelli M, Husvogt L, Vardeh H, Faulkner-Jones BE, Hornegger J, Connolly JL, Fujimoto JG (2016)
Publication Language: English
Publication Type: Journal article, Original article
Publication year: 2016
Journal
Publisher: Public Library of Science
Book Volume: 11
Pages Range: 1-13
Journal Issue: 8
URI: https://www5.informatik.uni-erlangen.de/Forschung/Publikationen/2016/Giacomelli16-VHA.pdf
DOI: 10.1371/journal.pone.0159337
Open Access Link: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0159337
Abstract
We derive a physically realistic model for the generation of virtual transillumination, white light microscopy images using epi-fluorescence measurements from thick, unsectioned tissue. We demonstrate this technique by generating virtual transillumination H&E images of unsectioned human breast tissue from epi-fluorescence multiphoton microscopy data. The virtual transillumination algorithm is shown to enable improved contrast and color accuracy compared with previous color mapping methods. Finally, we present an open source implementation of the algorithm in OpenGL, enabling real-time GPU-based generation of virtual transillumination microscopy images using conventional fluorescence microscopy systems.
Authors with CRIS profile
Lennart Husvogt
Lehrstuhl für Informatik 5 (Mustererkennung)
Joachim Hornegger
Lehrstuhl für Informatik 14 (Bild- und Sprachverarbeitung)
Involved external institutions
Massachusetts Institute of Technology (MIT)
United States (USA) (US)
Veterans Affairs Healthcare System Boston and Harvard Medical School
United States (USA) (US)
Harvard University
United States (USA) (US)
University of Rochester Medical Center Rochester, NY
United States (USA) (US)
Beth Israel Deaconess Medical Center (BIDMC)
United States (USA) (US)
United States (USA) (US)
Veterans Affairs Healthcare System Boston and Harvard Medical School
United States (USA) (US)
Harvard University
United States (USA) (US)
University of Rochester Medical Center Rochester, NY
United States (USA) (US)
Beth Israel Deaconess Medical Center (BIDMC)
United States (USA) (US)
How to cite
APA:
Giacomelli, M., Husvogt, L., Vardeh, H., Faulkner-Jones, B.E., Hornegger, J., Connolly, J.L., & Fujimoto, J.G. (2016). Virtual Hematoxylin and Eosin Transillumination Microscopy Using Epi-Fluorescence Imaging. PLoS ONE, 11(8), 1-13. https://doi.org/10.1371/journal.pone.0159337
MLA:
Giacomelli, Michael, et al. "Virtual Hematoxylin and Eosin Transillumination Microscopy Using Epi-Fluorescence Imaging." PLoS ONE 11.8 (2016): 1-13.
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