Influence of different irradiation protocols on vascularization and bone formation parameters in rat femora.

Rottensteiner-Brandl U, Distel L, Stumpf M, Fey T, Köhn K, Bertram U, Lingens L, Greil P, Horch RE, Arkudas A (2017)

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2017

Journal

Tissue Engineering - Part C: Methods

Book Volume: 23

Pages Range: 583-591

Journal Issue: 10

DOI: 10.1089/ten.TEC.2017.0170

Abstract

INTRODUCTION Aim of the present study was the establishment of an efficient and reproducible model for irradiation of rat femora as a model for impaired osteogenesis and angiogenesis. MATERIALS AND METHODS Four different irradiation protocols were compared: single irradiation of the left femur with 20 Gy and explantation after 4 or 8 weeks (group A, B); three irradiation fractions at 3-4 days intervals with 10 Gy and explantation after 4 or 8 weeks (group C, D). The contralateral, unirradiated femur served as control. Evaluation included histology, microcomputertomography (μCT) and real time PCR. RESULTS Histology showed a pronounced increase of vacuoles in bone marrow after irradiation, especially after 4 weeks (group A and C), demonstrating bone marrow edema and fatty degeneration. Irradiation provoked a decrease of total cell numbers in cortical bone and of Hif1α positive cells in bone marrow. The expression of several markers (osteocalcin, RUNX2, TGFβ1, TNFα, VEGF-A, Hif1α) was decreased in group A after irradiation. This might suggest a decreased metabolism after irradiation. A significant decrease in small-sized vessels was seen in μCT evaluation in group A and D. CONCLUSIONS Single irradiation with 20 Gy had the most severe and reproducible impact on osteogenesis and angiogenesis after 4 weeks while being well tolerated by all animals, thus making it an excellent model for evaluation of bone healing and vascularization in irradiated tissue.

Authors with CRIS profile

Ulrike Rottensteiner-Brandl Lehrstuhl für Biochemie und Molekulare Medizin Luitpold Distel Medizinische Fakultät Martin Stumpf Lehrstuhl für Glas und Keramik (WW3) Tobias Fey Lehrstuhl für Glas und Keramik (WW3) Katrin Köhn Department of Plastic and Hand Surgery Ulf Bertram Department of Plastic and Hand Surgery Lara Lingens Department of Plastic and Hand Surgery Peter Greil Lehrstuhl für Glas und Keramik (WW3) Raymund Horch Professur für Plastische Chirurgie und Handchirurgie Andreas Arkudas Medizinische Fakultät

How to cite

APA:

Rottensteiner-Brandl, U., Distel, L., Stumpf, M., Fey, T., Köhn, K., Bertram, U.,... Arkudas, A. (2017). Influence of different irradiation protocols on vascularization and bone formation parameters in rat femora. Tissue Engineering - Part C: Methods, 23(10), 583-591. https://doi.org/10.1089/ten.TEC.2017.0170

MLA:

Rottensteiner-Brandl, Ulrike, et al. "Influence of different irradiation protocols on vascularization and bone formation parameters in rat femora." Tissue Engineering - Part C: Methods 23.10 (2017): 583-591.

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