Efficient and safe gene delivery to human corneal endothelium using magnetic nanoparticles

Czugala M, Mykhaylyk O, Boehler P, Onderka J, Stork B, Wesselborg S, Kruse F, Plank C, Singer BB, Fuchsluger T (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Nanomedicine Future Medicine

Book Volume: 11

Pages Range: 1787-800

Journal Issue: 14

DOI: 10.2217/nnm-2016-0144

Abstract

To develop a safe and efficient method for targeted, anti-apoptotic gene therapy of corneal endothelial cells (CECs).Magnetofection (MF), a combination of lipofection with magnetic nanoparticles (MNPs; PEI-Mag2, SO-Mag5, PalD1-Mag1), was tested in human CECs and in explanted human corneas. Effects on cell viability and function were investigated. Immunocompatibility was assessed in human peripheral blood mononuclear cells.Silica iron-oxide MNPs (SO-Mag5) combined with X-tremeGENE-HP achieved high transfection efficiency in human CECs and explanted human corneas, without altering cell viability or function. Magnetofection caused no immunomodulatory effects in human peripheral blood mononuclear cells. Magnetofection with anti-apoptotic P35 gene effectively blocked apoptosis in CECs.Magnetofection is a promising tool for gene therapy of corneal endothelial cells with potential for targeted on-site delivery.

Authors with CRIS profile

Friedrich Kruse Lehrstuhl für Augenheilkunde Thomas Fuchsluger Professur für Augenheilkunde (Cornea/vorderer Augenabschnitt)

Involved external institutions

Technische Universität München (TUM) DE Germany (DE) Heinrich-Heine-Universität Düsseldorf DE Germany (DE) Universität Duisburg-Essen (UDE) DE Germany (DE)

How to cite

APA:

Czugala, M., Mykhaylyk, O., Boehler, P., Onderka, J., Stork, B., Wesselborg, S.,... Fuchsluger, T. (2016). Efficient and safe gene delivery to human corneal endothelium using magnetic nanoparticles. Nanomedicine, 11(14), 1787-800. https://doi.org/10.2217/nnm-2016-0144

MLA:

Czugala, Marta, et al. "Efficient and safe gene delivery to human corneal endothelium using magnetic nanoparticles." Nanomedicine 11.14 (2016): 1787-800.

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