Corneal tissue interactions of a new 345 nm ultraviolet femtosecond laser

Hammer CM, Petsch C, Klenke J, Skerl K, Paulsen F, Kruse F, Seiler T, Menzel-Severing J (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Journal of Cataract and Refractive Surgery Elsevier

Book Volume: 41

Pages Range: 1279-88

Journal Issue: 6

DOI: 10.1016/j.jcrs.2014.11.046

Abstract

To assess the suitability of a new 345 nm ultraviolet (UV) femtosecond laser for refractive surgery.Department of Ophthalmology, University of Erlangen-Nürnberg, Erlangen, Germany.Experimental study.Twenty-five porcine corneas were used for stromal flap or lamellar bed creation (stromal depth, 150 ?m) and 15 rabbit corneas for lamellar bed creation near the endothelium. Ultraviolet femtosecond laser cutting-line morphology, gas formation, and keratocyte death rate were evaluated using light and electron microscopy and compared with a standard infrared (IR) femtosecond laser. Endothelial cell survival was examined after application of a laser cut near the endothelium.Flaps created by the UV laser were lifted easily. Gas formation was reduced 4.2-fold compared with the IR laser (P = .001). The keratocyte death rate near the interface was almost doubled; however, the death zone was confined to a region within 38 ?m ± 10 (SD) along the cutting line. Histologically and ultrastructurally, a distinct and continuous cutting line was not found after UV femtosecond laser application if flap lifting was omitted and standard energy parameters were used. Instead, a regular pattern of vertical striations, presumably representing self-focusing induced regions of optical tissue breakdown, were identified. Lamellar bed creation with standard energy parameters 50 ?m from the endothelium rendered the endothelial cells intact and viable.The new 345 nm femtosecond laser is a candidate for pending in vivo trials and future high-precision flap creation, intrastromal lenticule extraction, and ultrathin Descemet-stripping endothelial keratoplasty.Mr. Klenke and Ms. Skerl were paid employees of Wavelight GmbH when the study was performed. Dr. Seiler is a scientific consultant to Wavelight GmbH. No other author has a financial or proprietary interest in any material or method mentioned.

Authors with CRIS profile

Friedrich Paulsen Lehrstuhl für Funktionelle und Klinische Anatomie Friedrich Kruse Lehrstuhl für Augenheilkunde Johannes Menzel-Severing Department of Ophthalmology

Involved external institutions

WaveLight GmbH DE Germany (DE) IROC AG - Institut für Refraktive und Ophthalmo-Chirurgie CH Switzerland (CH)

How to cite

APA:

Hammer, C.M., Petsch, C., Klenke, J., Skerl, K., Paulsen, F., Kruse, F.,... Menzel-Severing, J. (2015). Corneal tissue interactions of a new 345 nm ultraviolet femtosecond laser. Journal of Cataract and Refractive Surgery, 41(6), 1279-88. https://doi.org/10.1016/j.jcrs.2014.11.046

MLA:

Hammer, Christian M., et al. "Corneal tissue interactions of a new 345 nm ultraviolet femtosecond laser." Journal of Cataract and Refractive Surgery 41.6 (2015): 1279-88.

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