Batf3 deficiency proves the pivotal role of CD8?(+) dendritic cells in protection induced by vaccination with attenuated Plasmodium sporozoites

Montagna GN, Biswas A, Hildner K, Matuschewski K, Dunay IR (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Parasite Immunology Wiley-Blackwell

DOI: 10.1111/pim.12222

Abstract

Increasing evidence indicates that hepatic CD8?(+) dendritic cells (DCs) are important antigen cross-presenting cells (APC) involved in the priming of protective CD8(+) T cell responses induced by live-attenuated Plasmodium sporozoites. Experimental proof for a critical role of CD8?(+) DCs in protective pre-erythrocytic malaria immunizations has pivotal implications for vaccine development, including improved vectored subunit vaccines. Employing Batf3(-/-) mice, which lack functional CD8?(+) DCs, we demonstrate that deficiency of these particular APCs completely abolishes protection and corresponding signatures of vaccine-induced immunity. We show that in wild-type, but not in Batf3(-/-) , mice CD8?(+) DCs accumulate in the liver after immunization with live irradiation-attenuated P. berghei sporozoites. IFN-? production by Plasmodium antigen-specific CD8(+) T cells is dependent on functional Batf3. In addition, our results demonstrate that the dysfunctional cDC-CD8+ T cell axis correlates with MHC class II upregulation on splenic CD8?(-) DCs. Collectively, these findings underscore the essential role of CD8?(+) DCs in robust protection induced by experimental live-attenuated malaria vaccines. This article is protected by copyright. All rights reserved.

Authors with CRIS profile

Kai Hildner Juniorprofessur für Pneumologie/ Immunologie

Involved external institutions

Otto-von-Guericke-Universität Magdeburg DE Germany (DE) Max-Planck-Institut für Infektionsbiologie / Max Planck Institute for Infection Biology DE Germany (DE)

How to cite

APA:

Montagna, G.N., Biswas, A., Hildner, K., Matuschewski, K., & Dunay, I.R. (2015). Batf3 deficiency proves the pivotal role of CD8?(+) dendritic cells in protection induced by vaccination with attenuated Plasmodium sporozoites. Parasite Immunology. https://doi.org/10.1111/pim.12222

MLA:

Montagna, G. N., et al. "Batf3 deficiency proves the pivotal role of CD8?(+) dendritic cells in protection induced by vaccination with attenuated Plasmodium sporozoites." Parasite Immunology (2015).

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