Pollen Tube Development

Johnson MA, Kost B (2010)

Publication Type: Book chapter / Article in edited volumes

Publication year: 2010

Publisher: Humana Press

Edited Volumes: Plant Developmental Biology

Series: Methods in Molecular Biology

City/Town: Totowa

Book Volume: 655

Pages Range: 155-176

ISBN: 978-1-60761-764-8

DOI: 10.1007/978-1-60761-765-5_11


Pollen tubes grow rapidly in a strictly polarized manner as they transport male reproductive cells through female flower tissues to bring about fertilization. Vegetative pollen tube cells are an excellent model system to investigate processes underlying directional cell expansion. In this chapter, we describe materials and methods required for (1) the identification of novel factors essential for polarized cell growth through the isolation and analysis of Arabidopsis mutants with defects in pollen tube growth and (2) the detailed functional characterization of pollen tube proteins based on transient transformation and microscopic analysis of cultured tobacco pollen tubes.

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Johnson, M.A., & Kost, B. (2010). Pollen Tube Development. In Hennig, Lars, Köhler, Claudia (Eds.) (Eds.), Plant Developmental Biology. (pp. 155-176). Totowa: Humana Press.


Johnson, Mark A., and Benedikt Kost. "Pollen Tube Development." Plant Developmental Biology. Ed. Hennig, Lars, Köhler, Claudia (Eds.), Totowa: Humana Press, 2010. 155-176.

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